Zebrafish YAC, BAG, and PAC genomic libraries

Chris T. Amemiya, Tao P. Zhong, Gary A. Silverman, Mark C. Fishman, Leonard I. Zon

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Numerous positional cloning projects directed at isolating genes responsible for the myriads of observed developmental defects in the zebrafish are anticipated in the very near future. In this chapter, we have reviewed the YAC, BAC, and PAC large-insert genomic resources available to the zebrafish community. We have discussed how these resources are screened and used in a positional cloning scheme and have pointed out frequently formidable logistical considerations in the approach. Despite being extremely tedious, positional cloning projects in the zebrafish will be comparatively easier to accomplish than in human and mouse, because of unique biological advantages of the zebrafish system. Moreover, the ease and speed at which genes are identified and cloned should rapidly increase as more mapping reagents and information become available, thereby paving the way for meaningful biological studies.

Original languageEnglish
Pages (from-to)235-258
Number of pages24
JournalMethods in cell biology
Issue number60
StatePublished - Dec 1 1999
Externally publishedYes

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    Amemiya, C. T., Zhong, T. P., Silverman, G. A., Fishman, M. C., & Zon, L. I. (1999). Zebrafish YAC, BAG, and PAC genomic libraries. Methods in cell biology, (60), 235-258.