@article{1d51b6780b20456a81bd90e8498d60a9,
title = "Zebrafish Retinal Ganglion Cells Asymmetrically Encode Spectral and Temporal Information across Visual Space",
abstract = "In vertebrate vision, the tetrachromatic larval zebrafish permits non-invasive monitoring and manipulating of neural activity across the nervous system in vivo during ongoing behavior. However, despite a perhaps unparalleled understanding of links between zebrafish brain circuits and visual behaviors, comparatively little is known about what their eyes send to the brain via retinal ganglion cells (RGCs). Major gaps in knowledge include any information on spectral coding and information on potentially critical variations in RGC properties across the retinal surface corresponding with asymmetries in the statistics of natural visual space and behavioral demands. Here, we use in vivo two-photon imaging during hyperspectral visual stimulation as well as photolabeling of RGCs to provide a functional and anatomical census of RGCs in larval zebrafish. We find that RGCs{\textquoteright} functional and structural properties differ across the eye and include a notable population of UV-responsive On-sustained RGCs that are only found in the acute zone, likely to support visual prey capture of UV-bright zooplankton. Next, approximately half of RGCs display diverse forms of color opponency, including many that are driven by a pervasive and slow blue-Off system—far in excess of what would be required to satisfy traditional models of color vision. In addition, most information on spectral contrast was intermixed with temporal information. Taken together, our results suggest that zebrafish RGCs send a diverse and highly regionalized time-color code to the brain.",
keywords = "2P imaging, UV-vision, color vision, larval zebrafish, prey capture, retina, retinal ganglion cells",
author = "Mingyi Zhou and John Bear and Roberts, {Paul A.} and Janiak, {Filip K.} and Julie Semmelhack and Takeshi Yoshimatsu and Tom Baden",
note = "Funding Information: We thank Daniel Osorio and Katrin Franke for critical feedback, Philipp Berens for advising on statistical analyses, and Maxime Zimmermann for providing the 3D zebrafish model. The authors would also like to acknowledge support from the FENS-Kavli Network of Excellence and the EMBO YIP . Funding was provided from the European Research Council (ERC-StG “NeuroVisEco” 677687 to T.B.), the UKRI (BBSRC, BB/R014817/1 and MRC, MC_PC_15071 to T.B.), the Leverhulme Trust ( PLP-2017-005 to T.B.), the Lister Institute of Preventive Medicine (to T.B.), the Marie Curie Sklodowska Actions individual fellowship (“ColourFish” 748716 to T.Y.) from the European Union{\textquoteright}s Horizon 2020 research and innovation programme , and the Sussex-HKUST joint PhD programme (to T.B. and J.S.). Funding Information: We thank Daniel Osorio and Katrin Franke for critical feedback, Philipp Berens for advising on statistical analyses, and Maxime Zimmermann for providing the 3D zebrafish model. The authors would also like to acknowledge support from the FENS-Kavli Network of Excellence and the EMBO YIP. Funding was provided from the European Research Council (ERC-StG ?NeuroVisEco? 677687 to T.B.), the UKRI (BBSRC, BB/R014817/1 and MRC, MC_PC_15071 to T.B.), the Leverhulme Trust (PLP-2017-005 to T.B.), the Lister Institute of Preventive Medicine (to T.B.), the Marie Curie Sklodowska Actions individual fellowship (?ColourFish? 748716 to T.Y.) from the European Union's Horizon 2020 research and innovation programme, and the Sussex-HKUST joint PhD programme (to T.B. and J.S.). M.Z. J.B. T.Y. and T.B. designed the study, with input from all authors; M.Z. J.B. and T.Y. generated novel transgenic lines; F.K.J. developed custom two-photon scan options; M.Z. and J.B. performed functional recordings and data pre-processing: population recordings around the eye, single cell recordings, comparison of flat versus natural stats noise stimulation (M.Z.), and population recordings in the SZ (J.B.); J.B. performed paGFP experiments and retinal cell counts and data pre-processing; T.Y. computed cell-density projections into visual space; P.A.R. performed clustering analyses and dendritic tilt quantification; and T.B. performed additional data analysis and wrote the manuscript, with input from all authors. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2020 The Author(s)",
year = "2020",
month = aug,
day = "3",
doi = "10.1016/j.cub.2020.05.055",
language = "English",
volume = "30",
pages = "2927--2942.e7",
journal = "Current Biology",
issn = "0960-9822",
number = "15",
}