TY - JOUR
T1 - Yeast artificial chromosome cloning of a two-megabase-size contig within chromosomal band 18q21 establishes physical linkage between BCL2 and plasminogen activator inhibitor type-2
AU - Silverman, Gary A.
AU - Jockel, Jennifer I.
AU - Domer, Peter H.
AU - Mohr, Rose M.
AU - Taillon-Miller, Patricia
AU - Korsmeyer, Stanley J.
N1 - Funding Information:
We gratefully acknowledge Drs. Maynard Olson, Helen Donis-Keller, and David Schlessinger for helpful discussions and Dr. Bernard Brownstein and the Center for Genetics in Medicine for screening the YAC library. We are indebted to Mrs. Carolyn Dav-inroy for preparation of this manuscript. This work was supported by NIH Grant 1 ROl CA 60239-01. G.A.S. was support,ed by a National Research Service Award (HD07Z71) from the National Institute of Child Health and Human Development.
PY - 1991/2
Y1 - 1991/2
N2 - The construction of large-scale physical maps requires efficient approaches to generate new probes and link informative markers. The mapping of a human chromosomal segment was initiated by using the 18q21.3 probes, plasminogen activator inhibitor type-2 (PLANH2) and BCL2, to screen a yeast artificial chromosome (YAC) library. An inverse polymerase chain reaction technique rescued genomic ends of the YAC inserts. These new probes were used in a chromosomal walking strategy which established that the PLANH2 gene was 600 kb telomeric and in the opposite transcriptional orientation to that of BCL2. Overall, 16 YACs with a mean size of ∼300 kb were analyzed using rare-cutting restriction endonucleases and 10 end-rescued probes. A contiguous map within 18q21.3 that spans approximately 2 Mb was assembled. This establishes the feasibility of using YACs in the efficient cloning and physical surveying of expanses of the human genome lacking closely spaced, genetic landmarks.
AB - The construction of large-scale physical maps requires efficient approaches to generate new probes and link informative markers. The mapping of a human chromosomal segment was initiated by using the 18q21.3 probes, plasminogen activator inhibitor type-2 (PLANH2) and BCL2, to screen a yeast artificial chromosome (YAC) library. An inverse polymerase chain reaction technique rescued genomic ends of the YAC inserts. These new probes were used in a chromosomal walking strategy which established that the PLANH2 gene was 600 kb telomeric and in the opposite transcriptional orientation to that of BCL2. Overall, 16 YACs with a mean size of ∼300 kb were analyzed using rare-cutting restriction endonucleases and 10 end-rescued probes. A contiguous map within 18q21.3 that spans approximately 2 Mb was assembled. This establishes the feasibility of using YACs in the efficient cloning and physical surveying of expanses of the human genome lacking closely spaced, genetic landmarks.
UR - http://www.scopus.com/inward/record.url?scp=0026032014&partnerID=8YFLogxK
U2 - 10.1016/0888-7543(91)90245-A
DO - 10.1016/0888-7543(91)90245-A
M3 - Article
C2 - 2004771
AN - SCOPUS:0026032014
SN - 0888-7543
VL - 9
SP - 219
EP - 228
JO - Genomics
JF - Genomics
IS - 2
ER -