Y-box Binding Protein-1 Is Part of a Complex Molecular Network Linking ΔNp63α to the PI3K/akt Pathway in Cutaneous Squamous Cell Carcinoma

Annaelena Troiano, Irene Schiano Lomoriello, Orsola di Martino, Sabato Fusco, Alessandra Pollice, Maria Vivo, Girolama La Mantia, Viola Calabrò

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Cutaneous squamous cell carcinomas (SCCs) typically lack somatic oncogene-activating mutations and most of them contain p53 mutations. However, the presence of p53 mutations in skin premalignant lesions suggests that these represent early events during tumor progression and additional alterations may be required for SCC development. SCC cells frequently express high levels of ΔNp63α and Y-box binding 1 (YB-1 or YBX1) oncoproteins. Here, we show that knockdown of YB-1 in spontaneously immortalized HaCaT and non-metastatic SCC011 cells led to a dramatic decrease of ΔNp63α, cell detachment and death. In highly metastatic SCC022 cells, instead, YB-1 silencing induces PI3K/AKT signaling hyperactivation which counteracts the effect of YB-1 depletion and promotes cell survival. In summary, our results unveil a functional cross-talk between YB-1, ΔNp63α and the PI3K/AKT pathway critically governing survival of squamous carcinoma cells.

Original languageEnglish
Pages (from-to)2067-2074
Number of pages8
JournalJournal of Cellular Physiology
Volume230
Issue number9
DOIs
StatePublished - Sep 1 2015

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