TY - JOUR
T1 - Xenopus laevis histone genes
T2 - Variant H1 genes are present in different clusters
AU - Zernik, Maria
AU - Heintz, Nathaniel
AU - Boime, Irving
AU - Roeder, Robert G.
N1 - Funding Information:
These studies were supported by research grants from the National Cancer Institute and the American Cancer Society. R.G.R. is a Camille and Henry Dreyfus Teacher-Scholar Awardee. I.B. is a recipient of an NIH Research Career Development Award. N.H. is a Damon Runyon-Walter Winchell Cancer Fund postdoctoral fellow. We acknowledge W. Wahli and I. Dawid for their generous gift of the X. laevis genomic DNA library. We thank Mark Boothby for helpful discussions and comments during the course of this work.
PY - 1980/12
Y1 - 1980/12
N2 - The Xenopus laevis histone genes have been isolated from a bacteriophage λ library using a cloned cDNA probe to X. laevis H4 mRNA (pX1ch4). Their structural organization has been determined by restriction mapping, blot hybridization and hybridization selection and translation of Xenopus histone mRNAs. They are clustered and probably tandemly arranged but, in contrast to invertebrate histone genes (Kedes, 1979), there has been extensive sequence divergence in the spacer regions of the clusters. Also, the order of the genes within individual clusters is not conserved. We report the isolation of two variant histone H1 genes and find that the order of the nucleosomal core histone genes within a cluster containing an H1B gene is different from that found in two clusters containing the H1A gene.
AB - The Xenopus laevis histone genes have been isolated from a bacteriophage λ library using a cloned cDNA probe to X. laevis H4 mRNA (pX1ch4). Their structural organization has been determined by restriction mapping, blot hybridization and hybridization selection and translation of Xenopus histone mRNAs. They are clustered and probably tandemly arranged but, in contrast to invertebrate histone genes (Kedes, 1979), there has been extensive sequence divergence in the spacer regions of the clusters. Also, the order of the genes within individual clusters is not conserved. We report the isolation of two variant histone H1 genes and find that the order of the nucleosomal core histone genes within a cluster containing an H1B gene is different from that found in two clusters containing the H1A gene.
UR - http://www.scopus.com/inward/record.url?scp=0019195487&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(80)90557-7
DO - 10.1016/0092-8674(80)90557-7
M3 - Article
C2 - 7460012
AN - SCOPUS:0019195487
SN - 0092-8674
VL - 22
SP - 807
EP - 815
JO - Cell
JF - Cell
IS - 3
ER -