XBP1 activates the transcription of its target genes via an ACGT core sequence under ER stress

Soshi Kanemoto, Shinichi Kondo, Maiko Ogata, Tomohiko Murakami, Fumihiko Urano, Kazunori Imaizumi

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

In mammals, the transmembrane protein kinase/endoribonuclease IRE1 is activated by endoplasmic reticulum stress and subsequently processes XBP1 mRNA to generate an active form of XBP1 protein. The spliced form of XBP1 protein acts as a transcription factor and induces the expression of ER-resident molecular chaperones. However, the mechanism for how XBP1 promotes the transcription of its target genes as well as the cis-acting elements for XBP1 during ER stress has been unclear. Recently, it was demonstrated that the expression of MDG1/ERdj4, a member of the DnaJ family, is regulated by the IRE1-XBP1 pathway. In the present report, we investigated the regulatory mechanisms of MDG1/ERdj4 gene expression by XBP1. We identified a cis-acting element in the MDG1/ERdj4 promoter region, to which XBP1 specifically binds in response to ER stress. Our results reveal a target sequence for the IRE1-XBP1 pathway under ER stress conditions.

Original languageEnglish
Pages (from-to)1146-1153
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume331
Issue number4
DOIs
StatePublished - Jun 17 2005
Externally publishedYes

Keywords

  • ACGT core
  • ER stress
  • IRE1
  • Transcription
  • UPR
  • XBP1
  • cis-element

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