520 Scopus citations

Abstract

Type 1 pili - adhesive fibers expressed in most members of the Enterobacteriaceae family - mediate binding to mannose receptors on host cells through the FimH adhesin. Pilus biogenesis proceeds by way of the chaperone/usher pathway. The x-ray structure of the FimC-FimH chaperone- adhesin complex from uropathogenic Escherichia coli at 2.5 angstrom resolution reveals the basis for carbohydrate recognition and for pilus assembly. The carboxyl-terminal pilin domain of FimH has an immunoglobulin- like ford, except that the seventh strand is missing, leaving part of the hydrophobic core exposed. A donor strand complementation mechanism in which the chaperone donates a strand to complete the pilin domain explains the basis for both chaperone function and pilus biogenesis.

Original languageEnglish
Pages (from-to)1061-1066
Number of pages6
JournalScience
Volume285
Issue number5430
DOIs
StatePublished - Aug 13 1999

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