Wld S but not Nmnat1 protects dopaminergic neurites from MPP + neurotoxicity

Jo Ann V. Antenor-Dorsey, Karen L. O'Malley

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Background: The Wld S mouse mutant ("Wallerian degeneration-slow") delays axonal degeneration in a variety of disorders including in vivo models of Parkinson's disease. The mechanisms underlying Wld S -mediated axonal protection are unclear, although many studies have attributed Wld S neuroprotection to the NAD +- synthesizing Nmnat1 portion of the fusion protein. Here, we used dissociated dopaminergic cultures to test the hypothesis that catalytically active Nmnat1 protects dopaminergic neurons from toxin-mediated axonal injury. Results: Using mutant mice and lentiviral transduction of dopaminergic neurons, the present findings demonstrate that Wld S but not Nmnat1, Nmnat3, or cytoplasmically-targeted Nmnat1 protects dopamine axons from the parkinsonian mimetic N-methyl-4-phenylpyridinium (MPP +). Moreover, NAD + synthesis is not required since enzymatically-inactive Wld S still protects. In addition, NAD + by itself is axonally protective and together with Wld S is additive in the MPP + model. Conclusions: Our data suggest that NAD + and Wld S act through separate and possibly parallel mechanisms to protect dopamine axons. As MPP + is thought to impair mitochondrial function, these results suggest that Wld S might be involved in preserving mitochondrial health or maintaining cellular metabolism.

Original languageEnglish
Article number5
JournalMolecular neurodegeneration
Volume7
Issue number1
DOIs
StatePublished - 2012

Keywords

  • MPP
  • Nmnat1
  • Parkinson's disease
  • Wld
  • axonal degeneration
  • dopaminergic neurons

Fingerprint

Dive into the research topics of 'Wld <sup>S</sup> but not Nmnat1 protects dopaminergic neurites from MPP <sup>+</sup> neurotoxicity'. Together they form a unique fingerprint.

Cite this