TY - JOUR
T1 - Wireless Optofluidic Systems for Programmable In Vivo Pharmacology and Optogenetics
AU - Jeong, Jae Woong
AU - McCall, Jordan G.
AU - Shin, Gunchul
AU - Zhang, Yihui
AU - Al-Hasani, Ream
AU - Kim, Minku
AU - Li, Shuo
AU - Sim, Joo Yong
AU - Jang, Kyung In
AU - Shi, Yan
AU - Hong, Daniel Y.
AU - Liu, Yuhao
AU - Schmitz, Gavin P.
AU - Xia, Li
AU - He, Zhubin
AU - Gamble, Paul
AU - Ray, Wilson Z.
AU - Huang, Yonggang
AU - Bruchas, Michael R.
AU - Rogers, John A.
N1 - Funding Information:
This material is based on work supported by the EUREKA NIDA R01DA037152 (M.R.B.), NIMH F31 MH101956 (J.G.M.), and NIDA K99DA038725 (to R.A.). We thank the Bruchas laboratory and the laboratory of Dr. Robert W. Gereau IV, in particular Tayler Sheahan and Dr. Judith Golden (Washington University) for helpful discussion. We thank Dr. Karl Deisseroth (Stanford University) for the channelrhodopsin-2 (H134), Dr. Garret Stuber (UNC) for the TH-IRES-Cre mice, the WUSTL Hope Center Viral Vector Core for viral packaging, and the WUSTL Pain Center for use of the rotarod and running wheels. All biomedical aspects of the device work were supported by a National Security Science and Engineering Faculty Fellowship of Energy (J.A.R.). The LED development was enabled by funding from the US Department of Energy, Division of Materials Sciences under award number DE-FG02-07ER46471 (J.A.R.), the NIH Common Fund NINDS R01NS081707 (J.A.R. and M.R.B.), and through the Materials Research Laboratory and Center for Microanalysis of Materials ( DE-FG02-07ER46453 ) (J.A.R.).
Publisher Copyright:
©2015 Elsevier Inc.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - In vivo pharmacology and optogenetics hold tremendous promise for dissection of neural circuits, cellular signaling, and manipulating neurophysiological systems in awake, behaving animals. Existing neural interface technologies, such as metal cannulas connected to external drug supplies for pharmacological infusions and tethered fiber optics for optogenetics, are not ideal for minimally invasive, untethered studies on freely behaving animals. Here, we introduce wireless optofluidic neural probes that combine ultrathin, soft microfluidic drug delivery with cellular-scale inorganic light-emitting diode (μ-ILED) arrays. These probes are orders of magnitude smaller than cannulas and allow wireless, programmed spatiotemporal control of fluid delivery and photostimulation. We demonstrate these devices in freely moving animals to modify gene expression, deliver peptide ligands, and provide concurrent photostimulation with antagonist drug delivery to manipulate mesoaccumbens reward-related behavior. The minimally invasive operation of these probes forecasts utility in other organ systems and species, with potential for broad application in biomedical science, engineering, and medicine.
AB - In vivo pharmacology and optogenetics hold tremendous promise for dissection of neural circuits, cellular signaling, and manipulating neurophysiological systems in awake, behaving animals. Existing neural interface technologies, such as metal cannulas connected to external drug supplies for pharmacological infusions and tethered fiber optics for optogenetics, are not ideal for minimally invasive, untethered studies on freely behaving animals. Here, we introduce wireless optofluidic neural probes that combine ultrathin, soft microfluidic drug delivery with cellular-scale inorganic light-emitting diode (μ-ILED) arrays. These probes are orders of magnitude smaller than cannulas and allow wireless, programmed spatiotemporal control of fluid delivery and photostimulation. We demonstrate these devices in freely moving animals to modify gene expression, deliver peptide ligands, and provide concurrent photostimulation with antagonist drug delivery to manipulate mesoaccumbens reward-related behavior. The minimally invasive operation of these probes forecasts utility in other organ systems and species, with potential for broad application in biomedical science, engineering, and medicine.
UR - http://www.scopus.com/inward/record.url?scp=84938740931&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2015.06.058
DO - 10.1016/j.cell.2015.06.058
M3 - Article
C2 - 26189679
AN - SCOPUS:84938740931
SN - 0092-8674
VL - 162
SP - 662
EP - 674
JO - Cell
JF - Cell
IS - 3
ER -