Wide-field two-dimensional multifocal optical-resolution photoacoustic-computed microscopy

Jun Xia, Guo Li, Lidai Wang, Mohammadreza Nasiriavanaki, Konstantin Maslov, John A. Engelbach, Joel R. Garbow, Lihong V. Wang

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technique that directly images optical absorption in tissue at high spatial resolution. To date, the majority of OR-PAM systems are based on single-focused optical excitation and ultrasonic detection, limiting the wide-field imaging speed. While 1D multifocal OR-PAM (1D-MFOR-PAM) has been developed, the potential of microlens and transducer arrays has not been fully realized. Here we present the development of 2D multifocal optical-resolution photoacoustic-computed microscopy (2D-MFOR-PACM), using a 2D microlens array and a full-ring ultrasonic transducer array. The 10 mm × 10 mm microlens array generates 1800 optical foci within the focal plane of the 512-element transducer array, and raster scanning the microlens array yields optical-resolution photoacoustic images. The system has improved the in-plane resolution of a full-ring transducer array from =100 to 29 μm and achieved an imaging time of 36 s over a 10 mm × 10 mm field of view. In comparison, the 1D-MFOR-PAM would take more than 4 min to image over the same field of view. The imaging capability of the system was demonstrated on phantoms and animals both ex vivo and in vivo.

Original languageEnglish
Pages (from-to)5236-5239
Number of pages4
JournalOptics Letters
Volume38
Issue number24
DOIs
StatePublished - Dec 15 2013

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