Whole-rat conditional gene knockout via genome editing

Andrew J. Brown; Daniel A. Fisher; Evguenia Kouranova; Aaron McCoy; Kevin Forbes; Yumei Wu; Rachel Henry; Diana Ji; Andre Chambers; Joe Warren; Weiguo Shu; Edward J. Weinstein; Xiaoxia Cui

doi:10.1038/nmeth.2516

Whole-rat conditional gene knockout via genome editing

Andrew J. Brown, Daniel A. Fisher, Evguenia Kouranova, Aaron McCoy, Kevin Forbes, Yumei Wu, Rachel Henry, Diana Ji, Andre Chambers, Joe Warren, Weiguo Shu, Edward J. Weinstein, Xiaoxia Cui

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.

Original language	English
Pages (from-to)	638-640
Number of pages	3
Journal	Nature Methods
Volume	10
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.2516
State	Published - Jul 2013

Access to Document

10.1038/nmeth.2516

Cite this

@article{2a5a5b555b06408a9cd6065a5942c18f,

title = "Whole-rat conditional gene knockout via genome editing",

abstract = "Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.",

author = "Brown, {Andrew J.} and Fisher, {Daniel A.} and Evguenia Kouranova and Aaron McCoy and Kevin Forbes and Yumei Wu and Rachel Henry and Diana Ji and Andre Chambers and Joe Warren and Weiguo Shu and Weinstein, {Edward J.} and Xiaoxia Cui",

year = "2013",

month = jul,

doi = "10.1038/nmeth.2516",

language = "English",

volume = "10",

pages = "638--640",

journal = "Nature Methods",

issn = "1548-7091",

number = "7",

}

TY - JOUR

T1 - Whole-rat conditional gene knockout via genome editing

AU - Brown, Andrew J.

AU - Fisher, Daniel A.

AU - Kouranova, Evguenia

AU - McCoy, Aaron

AU - Forbes, Kevin

AU - Wu, Yumei

AU - Henry, Rachel

AU - Ji, Diana

AU - Chambers, Andre

AU - Warren, Joe

AU - Shu, Weiguo

AU - Weinstein, Edward J.

AU - Cui, Xiaoxia

PY - 2013/7

Y1 - 2013/7

N2 - Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.

AB - Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.

UR - http://www.scopus.com/inward/record.url?scp=84879686686&partnerID=8YFLogxK

U2 - 10.1038/nmeth.2516

DO - 10.1038/nmeth.2516

M3 - Article

C2 - 23749298

AN - SCOPUS:84879686686

SN - 1548-7091

VL - 10

SP - 638

EP - 640

JO - Nature Methods

JF - Nature Methods

IS - 7

ER -

Whole-rat conditional gene knockout via genome editing

Abstract

Access to Document

Other files and links

Fingerprint

Cite this