Whole-rat conditional gene knockout via genome editing

Andrew J. Brown, Daniel A. Fisher, Evguenia Kouranova, Aaron McCoy, Kevin Forbes, Yumei Wu, Rachel Henry, Diana Ji, Andre Chambers, Joe Warren, Weiguo Shu, Edward J. Weinstein, Xiaoxia Cui

Research output: Contribution to journalArticlepeer-review

60 Scopus citations

Abstract

Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.

Original languageEnglish
Pages (from-to)638-640
Number of pages3
JournalNature Methods
Volume10
Issue number7
DOIs
StatePublished - Jul 2013

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