Whole-mount immunostaining of the adult Drosophila gastrointestinal tract

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The gastrointestinal (GI) tract harbors an essential barrier epithelium that separates an organism from its changing external environment. As such, the gut epithelium is a fascinating nexus of stem cell biology, immunology and physiology. Investigators have sought to mine this rich interface for new biological and mechanistic insights. Many of the powerful genetic approaches developed in Drosophila have proven effective in the study of the gut. The goal of this article is to present a method for dissecting, immunostaining and mounting samples of the adult Drosophila GI tract. This protocol combines readily with techniques to label cell lineages and/or challenge the system with environmental perturbations, which are briefly discussed.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalMethods
Volume68
Issue number1
DOIs
StatePublished - Jun 15 2014

Keywords

  • Adult Drosophila midgut
  • Environmental challenge
  • Lineage tracing
  • Stem cell

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