TY - JOUR
T1 - Wax plants disentangled
T2 - A phylogeny of Hoya (Marsdenieae, Apocynaceae) inferred from nuclear and chloroplast DNA sequences
AU - Wanntorp, Livia
AU - Kocyan, Alexander
AU - Renner, Susanne S.
N1 - Funding Information:
We thank H. Turner (University of Leiden, The Netherlands) for help with the map, H-E. Wanntorp (Stockholm University) for the drawing of Hoya multiflora, M. Vosyka (University of Munich) for laboratory assistence, P.I. Forster (Queensland Herbarium), and T. Livshultz (Harvard University), and two anonymous reviewers for comments on the manuscript. Part of the molecular work was supported by grants to the first author from “Stiftelsen Lars Hiertas Minne” and from the Swedish Royal Academy of Sciences. This paper is part of a research project by the first author supported by the Swedish Research Council.
PY - 2006/6
Y1 - 2006/6
N2 - Hoya (Marsdenieae, Apocynaceae) includes at least 200 species distributed from India to the Pacific Islands. We here infer major species groups in the genus based on combined sequences from the chloroplast atpB-rbcL spacer, the trnL region, and nuclear ribosomal DNA ITS region for 42 taxa of Hoya and close relatives. To assess levels of ITS polymorphism, ITS sequences for a third of the accessions were obtained by cloning. Most ITS clones grouped by species, indicating that speciation in Hoya usually predates ITS duplication. One ITS sequence of H. carnosa, however, grouped with a sequence of the morphologically similar H. pubicalyx, pointing to recent hybridization or the persistence of paralogous copies through a speciation event. The topology resulting from the combined chloroplast and nuclear data recovers some morphology-based sections, such as Acanthostemma and Eriostemma, as well as a well-supported Australian/New Guinean clade. The combined data also suggest that morphological adaptations for ant-symbiosis evolved at least three times within Hoya.
AB - Hoya (Marsdenieae, Apocynaceae) includes at least 200 species distributed from India to the Pacific Islands. We here infer major species groups in the genus based on combined sequences from the chloroplast atpB-rbcL spacer, the trnL region, and nuclear ribosomal DNA ITS region for 42 taxa of Hoya and close relatives. To assess levels of ITS polymorphism, ITS sequences for a third of the accessions were obtained by cloning. Most ITS clones grouped by species, indicating that speciation in Hoya usually predates ITS duplication. One ITS sequence of H. carnosa, however, grouped with a sequence of the morphologically similar H. pubicalyx, pointing to recent hybridization or the persistence of paralogous copies through a speciation event. The topology resulting from the combined chloroplast and nuclear data recovers some morphology-based sections, such as Acanthostemma and Eriostemma, as well as a well-supported Australian/New Guinean clade. The combined data also suggest that morphological adaptations for ant-symbiosis evolved at least three times within Hoya.
KW - atpB-rbcL spacer
KW - Bayesian inference
KW - Chloroplast DNA
KW - Hoya
KW - Nuclear ribosomal DNA ITS region
KW - Paralogus
KW - Parsimony
KW - trnL region
UR - https://www.scopus.com/pages/publications/33646386914
U2 - 10.1016/j.ympev.2006.01.022
DO - 10.1016/j.ympev.2006.01.022
M3 - Article
C2 - 16515867
AN - SCOPUS:33646386914
SN - 1055-7903
VL - 39
SP - 722
EP - 733
JO - Molecular Phylogenetics and Evolution
JF - Molecular Phylogenetics and Evolution
IS - 3
ER -