Abstract
Ion exchangers with various capacities (0.1-0.2 mg-equiv/g of dry gel) are synthesized by means of immobilization of DNA in polyacrylamide gel. Exchanges of alkali metal cations and ammonium are studied on these exchangers and selectively coefficients are determined. The following selectivity series of immobilized DNA in reference to the above-mentioned cations is stated: Li+ greater than or equal to NH4+ greater than or equal to Cs+ greater than Rb+ greater than K+ greater than or equal to Na+. The peculiar properties of Li+ and NH4+ in this series are noted and a possible explanation of this fact is offered. A supposition regarding the reduced activity of water in the polyacrylamide gel containing DNA is made.
| Translated title of the contribution | Interaction of immobilized DNA with alkaline metal and ammonium ions |
|---|---|
| Original language | Russian |
| Pages (from-to) | 1583-1589 |
| Number of pages | 7 |
| Journal | Molekulyarnaya Biologiya |
| Volume | 18 |
| Issue number | 6 |
| State | Published - Nov 1 1984 |