Voltage dependent calcium channel expression in isolated osteoclasts.

A. Teti, M. Grano, S. Colucci, L. Argentino, R. Barattolo, A. Miyauchi, S. L. Teitelbaum, K. A. Hruska, A. Zambonin Zallone

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Abstract

In this study the expression of voltage-dependent calcium channels on osteoclast plasma membrane has been investigated. We found that osteoclasts were sensitive to KCl-induced depolarization. In this circumstance a 4 fold transient cytosolic calcium concentration ([Ca2+]i) increase was observed. This increase was dose-dependent. Its half maximal effect was achieved at 30 mM KCl. Voltage sensitive calcium channels in osteoclasts were inhibited by specific antagonists. Nicardipine, a dihydropyridine derivative, was the most effective, inducing complete block of the channels at 10(-6) M. Verapamil (phenylalkylamine) and diltiazem (benzodiazepine) were less effective. These results are consistent with the presence, on the osteoclast membrane, of L-type voltage-sensitive calcium channels.

Original languageEnglish
Pages (from-to)1115-1118
Number of pages4
JournalBollettino della Societa italiana di biologia sperimentale
Volume65
Issue number12
StatePublished - Dec 1 1989

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