Voltage dependent calcium channel expression in isolated osteoclasts.

A. Teti, M. Grano, S. Colucci, L. Argentino, R. Barattolo, A. Miyauchi, S. L. Teitelbaum, K. A. Hruska, A. Zambonin Zallone

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


In this study the expression of voltage-dependent calcium channels on osteoclast plasma membrane has been investigated. We found that osteoclasts were sensitive to KCl-induced depolarization. In this circumstance a 4 fold transient cytosolic calcium concentration ([Ca2+]i) increase was observed. This increase was dose-dependent. Its half maximal effect was achieved at 30 mM KCl. Voltage sensitive calcium channels in osteoclasts were inhibited by specific antagonists. Nicardipine, a dihydropyridine derivative, was the most effective, inducing complete block of the channels at 10(-6) M. Verapamil (phenylalkylamine) and diltiazem (benzodiazepine) were less effective. These results are consistent with the presence, on the osteoclast membrane, of L-type voltage-sensitive calcium channels.

Original languageEnglish
Pages (from-to)1115-1118
Number of pages4
JournalBollettino della Societa italiana di biologia sperimentale
Issue number12
StatePublished - Dec 1 1989


Dive into the research topics of 'Voltage dependent calcium channel expression in isolated osteoclasts.'. Together they form a unique fingerprint.

Cite this