TY - JOUR
T1 - Vitamin D metabolites specific binding by rat intestinal cytosol
AU - Haddad, J. H.
AU - Hahn, T. J.
AU - Birge, S. F.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1973/11/2
Y1 - 1973/11/2
N2 - The intestine of ricketic rats, a recognized target tissue of vitamin D, contains s soluble macromolecule capable of specific in vitro binding of both 25-hydroxycholecalciferol and 1,25-dihydroxycholecalciferol. Its sedimentation behavior on linear 5-20% sucrose gradients suggests as a molecular weight of approximately 100 000. The binding is specific for sterols possessing both an open B ring and 25-hydroxyl group, and is destroyed by pre-incubation with trypsin. The binding affinity for 25-hydroxycholecalciferol (KA = 2 · 109 1/mole) was 2.8 times that for 1,25-dihydroxycholecalciferol.
AB - The intestine of ricketic rats, a recognized target tissue of vitamin D, contains s soluble macromolecule capable of specific in vitro binding of both 25-hydroxycholecalciferol and 1,25-dihydroxycholecalciferol. Its sedimentation behavior on linear 5-20% sucrose gradients suggests as a molecular weight of approximately 100 000. The binding is specific for sterols possessing both an open B ring and 25-hydroxyl group, and is destroyed by pre-incubation with trypsin. The binding affinity for 25-hydroxycholecalciferol (KA = 2 · 109 1/mole) was 2.8 times that for 1,25-dihydroxycholecalciferol.
UR - http://www.scopus.com/inward/record.url?scp=0015897909&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(73)90011-1
DO - 10.1016/0304-4165(73)90011-1
M3 - Article
C2 - 4361568
AN - SCOPUS:0015897909
SN - 0304-4165
VL - 329
SP - 93
EP - 97
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 1
ER -