Vitamin D metabolites specific binding by rat intestinal cytosol

J. H. Haddad, T. J. Hahn, S. F. Birge

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The intestine of ricketic rats, a recognized target tissue of vitamin D, contains s soluble macromolecule capable of specific in vitro binding of both 25-hydroxycholecalciferol and 1,25-dihydroxycholecalciferol. Its sedimentation behavior on linear 5-20% sucrose gradients suggests as a molecular weight of approximately 100 000. The binding is specific for sterols possessing both an open B ring and 25-hydroxyl group, and is destroyed by pre-incubation with trypsin. The binding affinity for 25-hydroxycholecalciferol (KA = 2 · 109 1/mole) was 2.8 times that for 1,25-dihydroxycholecalciferol.

Original languageEnglish
Pages (from-to)93-97
Number of pages5
JournalBBA - General Subjects
Issue number1
StatePublished - Nov 2 1973


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