Visualizing microtubule structural transitions and interactions with associated proteins

Eva Nogales, Rui Zhang

Research output: Contribution to journalReview articlepeer-review

37 Scopus citations

Abstract

Microtubules (MTs) have been the subject of cryo-electron microscopy (cryo-EM) studies since the birth of this technique. Although MTs pose some unique challenges, having to do with the presence of a MT seam, lattice variability and disorder, MT cryo-EM reconstructions are steadily improving in resolution and providing exciting new insights into MT structure and function. Recent work has lead to the atomic-detail visualization of lateral contacts between tubulin subunits and the conformational changes that give rise to strain in the MT lattice accompanying GTP hydrolysis. Cryo-EM has also been invaluable in describing the interactions between MTs and MT associated proteins (MAPs), which function to regulate MT dynamic instability, move cargoes, or contribute to other MT cellular processes.

Original languageEnglish
Pages (from-to)90-96
Number of pages7
JournalCurrent Opinion in Structural Biology
Volume37
DOIs
StatePublished - Apr 1 2016

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