TY - JOUR
T1 - Visualizing microtubule structural transitions and interactions with associated proteins
AU - Nogales, Eva
AU - Zhang, Rui
N1 - Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Microtubules (MTs) have been the subject of cryo-electron microscopy (cryo-EM) studies since the birth of this technique. Although MTs pose some unique challenges, having to do with the presence of a MT seam, lattice variability and disorder, MT cryo-EM reconstructions are steadily improving in resolution and providing exciting new insights into MT structure and function. Recent work has lead to the atomic-detail visualization of lateral contacts between tubulin subunits and the conformational changes that give rise to strain in the MT lattice accompanying GTP hydrolysis. Cryo-EM has also been invaluable in describing the interactions between MTs and MT associated proteins (MAPs), which function to regulate MT dynamic instability, move cargoes, or contribute to other MT cellular processes.
AB - Microtubules (MTs) have been the subject of cryo-electron microscopy (cryo-EM) studies since the birth of this technique. Although MTs pose some unique challenges, having to do with the presence of a MT seam, lattice variability and disorder, MT cryo-EM reconstructions are steadily improving in resolution and providing exciting new insights into MT structure and function. Recent work has lead to the atomic-detail visualization of lateral contacts between tubulin subunits and the conformational changes that give rise to strain in the MT lattice accompanying GTP hydrolysis. Cryo-EM has also been invaluable in describing the interactions between MTs and MT associated proteins (MAPs), which function to regulate MT dynamic instability, move cargoes, or contribute to other MT cellular processes.
UR - http://www.scopus.com/inward/record.url?scp=84954543367&partnerID=8YFLogxK
U2 - 10.1016/j.sbi.2015.12.009
DO - 10.1016/j.sbi.2015.12.009
M3 - Review article
C2 - 26803284
AN - SCOPUS:84954543367
SN - 0959-440X
VL - 37
SP - 90
EP - 96
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
ER -