TY - JOUR
T1 - Virus-induced interferon α production by a dendritic cell subset in the absence of feedback signaling in vivo
AU - Barchet, Winfried
AU - Cella, Marina
AU - Odermatt, Bernhard
AU - Asselin-Paturel, Carine
AU - Colonna, Marco
AU - Kalinke, Ulrich
PY - 2002/2/18
Y1 - 2002/2/18
N2 - An effective type I interferon (IFN-α/β) response is critical for the control of many viral infections. Here we show that in vesicular stomatitis virus (VSV)-infected mouse embryonic fibroblasts (MEFs) the production of IFN-α is dependent on type I IFN receptor (IFNAR) triggering, whereas in infected mice early IFN-α production is IFNAR independent. In VSV-infected mice type I IFN is produced by few cells located in the marginal zone of the spleen. Unlike other dendritic cell (DC) subsets, FACS®-sorted CD11cintCD11b-GR-1+ DCs show high IFN-$ expression, irrespective of whether they were isolated from VSV-infected IFNAR-competent or -deficient mice. Thus, VSV preferentially activates a specialized DC subset presumably located in the marginal zone to produce high-level IFN-α largely independent of IFNAR feedback signaling.
AB - An effective type I interferon (IFN-α/β) response is critical for the control of many viral infections. Here we show that in vesicular stomatitis virus (VSV)-infected mouse embryonic fibroblasts (MEFs) the production of IFN-α is dependent on type I IFN receptor (IFNAR) triggering, whereas in infected mice early IFN-α production is IFNAR independent. In VSV-infected mice type I IFN is produced by few cells located in the marginal zone of the spleen. Unlike other dendritic cell (DC) subsets, FACS®-sorted CD11cintCD11b-GR-1+ DCs show high IFN-$ expression, irrespective of whether they were isolated from VSV-infected IFNAR-competent or -deficient mice. Thus, VSV preferentially activates a specialized DC subset presumably located in the marginal zone to produce high-level IFN-α largely independent of IFNAR feedback signaling.
KW - Dendritic cell subsets
KW - IFN regulatory factor 7
KW - IFN type I
KW - Type I IFN receptor
KW - Virus infection
UR - http://www.scopus.com/inward/record.url?scp=0037128177&partnerID=8YFLogxK
U2 - 10.1084/jem.20011666
DO - 10.1084/jem.20011666
M3 - Article
C2 - 11854363
AN - SCOPUS:0037128177
SN - 0022-1007
VL - 195
SP - 507
EP - 516
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 4
ER -