Virulent or avirulent (dhfr-ts-) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro

C. Brodskyn, S. M. Beverley, R. G. Titus

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

In this study we have compared the immune response of normal human cells cultured in vitro to two virulent strains of Leishmania major (CC1 and LV39), and to an avirulent vaccine strain (dhfr-ts-) made by targeted deletion of the essential gene DHFR-TS. We utilized an in vitro system in which naive T cells from normal human donors were primed with autologous Leishmania- infected macrophages. All three parasites infected macrophages and transformed into amastigotes within the cells. However, whereas LV39 and CC1 replicated in macrophages, dhfr-ts- did not. When peripheral blood lymphocytes (PBL) were stimulated with autologous macrophages infected with any of the three parasites, the lymphocytes produced a type-1-biased cytokine response. Finally, addition of IL-12 during the first stimulation period increased the production of interferon-gamma but decreased IL-5 secretion. On the other hand, anti-IL-12 resulted in the opposite effect.

Original languageEnglish
Pages (from-to)299-304
Number of pages6
JournalClinical and Experimental Immunology
Volume119
Issue number2
DOIs
StatePublished - 2000

Keywords

  • IL-12
  • Leishmania major
  • Macrophages
  • Th1
  • Th2

Fingerprint

Dive into the research topics of 'Virulent or avirulent (dhfr-ts-) Leishmania major elicit predominantly a type-1 cytokine response by human cells in vitro'. Together they form a unique fingerprint.

Cite this