Virulence gene expression in human community-acquired Staphylococcus aureus infection

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Isolates of methicillin-resistant Staphylococcus aureus (MRSA) were once linked uniformly with hospital-associated infections; however, community-acquired MRSA (CA-MRSA) now represents an emerging threat worldwide. To examine the association of differential virulence gene expression with outcomes of human infection, we measured transcript levels of target staphylococcal genes directly in clinical samples from children with active known or suspected CA-MRSA infections. Virulence genes encoding secreted toxins, including Panton-Valentine leukocidin, were highly expressed during superficial and invasive CA-MRSA infections. In contrast, increased expression of surface-associated protein A was linked only with invasive disease. Comparisons with laboratory-grown corresponding clinical isolates revealed that tissue-specific expression profiles reflect the activity of the staphylococcal accessory gene regulator during human infection. These results represent the first demonstration of staphylococcal gene expression and regulation directly in human tissue. Such analysis will help to unravel the complex interactions between CA-MRSA and its host environmental niches during disease development.

Original languageEnglish
Pages (from-to)294-301
Number of pages8
JournalJournal of Infectious Diseases
Volume199
Issue number3
DOIs
StatePublished - Feb 1 2009

Fingerprint Dive into the research topics of 'Virulence gene expression in human community-acquired Staphylococcus aureus infection'. Together they form a unique fingerprint.

  • Cite this