@article{27ccb6bd784641c9a5e3fadccb2aa829,
title = "Virome biogeography in the lower gastrointestinal tract of rhesus macaques with chronic diarrhea",
abstract = "The composition of gastrointestinal tract viromes has been associated with multiple diseases. Our understanding of virus communities in the GI tract is still very limited due to challenges in sampling from different GI sites. Here we defined the GI viromes of 15 rhesus macaques with chronic diarrhea. Luminal content samples from terminal ileum, proximal and distal colon were collected at necropsy while samples from the rectum were collected antemortem using a fecal loop. The composition of and ecological parameters associated with the terminal ileum virome were distinct from the colon and rectum samples; these differences were driven by bacteriophages rather than eukaryotic viruses. The six contigs that were most discriminative of the viromes were distantly related to bacteriophages from three different families. Our analysis provides support for using fecal loop sampling of the rectum as a proxy of the colonic virome in humans.",
keywords = "Bacteriophages, Biogeography, Chronic diarrhea, Gastrointestinal virome",
author = "Guoyan Zhao and Lindsay Droit and Gilbert, {Margaret H.} and Schiro, {Faith R.} and Didier, {Peter J.} and Xuemei Si and Anne Paredes and Handley, {Scott A.} and Virgin, {Herbert W.} and Bohm, {Rudolf P.} and David Wang",
note = "Funding Information: We would like to thank Brian Koebbe and Eric Martin from the High Throughput Computing Facility at the Center for Genome Sciences and Systems Biology for providing high-throughput computational resources and supports; Jessica Hoisington-Lopez from the DNA Sequencing Innovation Lab at the Center for Genome Sciences and Systems Biology for her sequencing expertise; This work was supported by National Institutes of Health Office of The Director, grants R24 OD019793 , National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) , National Institute of Allergy and Infectious Diseases (NIAID) and Washington University DDRCC Grant (NIDDK P30 DK052574 ). Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2019",
month = jan,
day = "15",
doi = "10.1016/j.virol.2018.10.001",
language = "English",
volume = "527",
pages = "77--88",
journal = "Virology",
issn = "0042-6822",
}