Viral Transduction of DRG Neurons

Research output: Contribution to journalArticle

Abstract

The manipulation of gene expression is an essential tool to study the function of genes or signaling pathways. Uniform and robust gene manipulation is crucial for successful assays. However, neuronal cells are generally difficult-to-transfect cells with conventional DNA/RNA transfection reagents. Therefore, virus-mediated gene delivery is a primary choice for the studies of gene functions in neurons. In this chapter, we will describe the methods for lentivirus-mediated gene expression or knockdown in DRG neurons.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume2143
DOIs
StatePublished - Jan 1 2020

Keywords

  • Axon degeneration
  • DRG
  • Lentivirus
  • Neurodegeneration
  • Wallerian degeneration

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