Viral infection and toll-like receptor agonists induce a differential expression of type I and λ interferons in humans plasmacytoid and monocyte-derived dendritic cells

Eliana M. Coccia, Martina Severa, Elena Giacomini, Daniéle Monneron, Maria Elena Remoli, Ilkka Julkunen, Marina Cella, Roberto Lande, Gilles Uzé

Research output: Contribution to journalArticlepeer-review

396 Scopus citations

Abstract

In humans, the type I interferon (IFN) family consists of 13 IFN-α subtypes, IFN-β and IFN-ω; the newly discovered IFN-like family consists of IFN-λ1, -λ2 and -λ3. We have investigated the expression of type I and λ IFN genes following virus infections or Toll-like receptor (TLR) triggering in monocyte-derived DC (MDDC) and plasmacytoid DC (pDC). We found that all IFN-α, -β, -ω and -λ subtypes are expressed in influenza-virus-infected MDDC or pDC. Conversely, differential type I IFN gene transcription was induced in MDDC and pDC stimulated by specific TLR agonists. TLR-9 stimulation by CpG DNA induced the expression of all IFN-α, -β, -ω and -λ subtypes in pDC, whereas TLR-4 stimulation by LPS, or TLR-3 stimulation by poly I:C, induced only IFN-β and IFN-λ gene expression in MDDC. The expression pattern of IFN regulatory factor (IRF)-5 and IRF-7 in MDDC and pDC was also determined. IRF-5 was constitutively expressed in the two DC subsets whereas IRF-7 was constitutive in pDC but its expression was induced along MDDC maturation. Overall, our data indicate that the coordinated expression of IFN-λ with IFN-β would be of crucial importance for the maturation of DC.

Original languageEnglish
Pages (from-to)796-805
Number of pages10
JournalEuropean Journal of Immunology
Volume34
Issue number3
DOIs
StatePublished - Mar 1 2004

Keywords

  • Cytokine
  • Dendritic cell
  • Interferon
  • Toll-like receptor
  • Viral infection

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