@article{339ec19b584f460fb72a5b58d7e362ba,
title = "Viral complementation of immunodeficiency confers protection against enteric pathogens via interferon-λ",
abstract = "Commensal microbes profoundly impact host immunity to enteric viral infections1. We have shown that the bacterial microbiota and host antiviral cytokine interferon-λ (IFN-λ) determine the persistence of murine norovirus in the gut2,3. However, the effects of the virome in modulating enteric infections remain unexplored. Here, we report that murine astrovirus can complement primary immunodeficiency to protect against murine norovirus and rotavirus infections. Protection against infection was horizontally transferable between immunocompromised mouse strains by co-housing and fecal transplantation. Furthermore, protection against enteric pathogens corresponded with the presence of a specific strain of murine astrovirus in the gut, and this complementation of immunodeficiency required IFN-λ signalling in gut epithelial cells. Our study demonstrates that elements of the virome can protect against enteric pathogens in an immunodeficient host.",
author = "Harshad Ingle and Sanghyun Lee and Teresa Ai and Anthony Orvedahl and Rachel Rodgers and Guoyan Zhao and Meagan Sullender and Peterson, {Stefan T.} and Marissa Locke and Liu, {Ta Chiang} and Yokoyama, {Christine C.} and Bridgett Sharp and Stacey Schultz-Cherry and Miner, {Jonathan J.} and Baldridge, {Megan T.}",
note = "Funding Information: We acknowledge all members of the Baldridge laboratory for helpful discussions. We also thank J. Hoisington-Lopez for assistance with sequencing, D. Kreamalmeyer for animal care and breeding, the Washington University Pulmonary Morphology core facility for assistance with histology, and J. White and the Washington University Central Gnotobiotic Facility for assistance with germ-free mice. We are grateful to the Estes laboratory for providing murine rotavirus. H.I. was supported by the Children{\textquoteright}s Discovery Institute of Washington University and a St Louis Children{\textquoteright}s Hospital Postdoctoral Research grant (MI-F-2018-712). S.L. was supported by the Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education (NRF-2016R1A6A3A03012352). A.O. was funded by the Pediatric Infectious Diseases Society/St Jude Children{\textquoteright}s Research Hospital Fellowship Program in Basic Research and NIH training grant T32AI106688. C.C.Y. was supported by NIH training grant T32AI007163. S.S.-C. was supported by ALSAC and NIH grant R03 AI126101-01. J.J.M. was supported by NIH grant K08 AR07091. M.T.B. was supported by NIH grants K22 AI127846, R01 AI127552, R01 AI139314 and R01 AI141478, Digestive Diseases Research Core Centers P30 DK052574, the Pew Biomedical Scholars Program and the Global Probiotics Council{\textquoteright}s Young Investigator Grant for Probiotics Research. Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2019",
month = jul,
day = "1",
doi = "10.1038/s41564-019-0416-7",
language = "English",
volume = "4",
pages = "1120--1128",
journal = "Nature Microbiology",
issn = "2058-5276",
number = "7",
}