Villous B cells of the small intestine are specialized for invariant NK T cell dependence

Peter Velázquez, Bo Wei, Michael McPherson, Lesley Marie A. Mendoza, Sandra L. Nguyen, Olga Turovskaya, Mitchell Kronenberg, Tiffany T. Huang, Matthew Schrage, Lynn N. Lobato, Daisuke Fujiwara, Sarah Brewer, Moshe Arditi, Genhong Cheng, R. Balfour Sartor, Rodney D. Newberry, Jonathan Braun

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


B cells are important in mucosal microbial homeostasis through their well-known role in secretory IgA production and their emerging role in mucosal immunoregulation. Several specialized intraintestinal B cell compartments have been characterized, but the nature of conventional B cells in the lamina propria is poorly understood. In this study, we identify a B cell population predominantly composed of surface IgM+ IgD+ cells residing in villi of the small intestine and superficial lamina propria of the large intestine, but distinct from the intraepithelial compartment or organized intestinal lymphoid structures. Small intestinal(villous) B cells are diminished in genotypes that alter the strength of BCR signaling(Bruton tyrosine kinasexid,Gαi2-/-), and in mice lacking cognate BCR specificity. They are not dependent on enteric microbial sensing, because they are abundant in mice that are germfree or genetically deficient in TLR signaling. However, villous B cells are reduced in the absence of invariant NK T cells(Jα18-/- or CD1d-/- mice). These findings define a distinct population of conventional B cells in small intestinal villi, and suggest an immunologic link between CDl-restricted invariant NK T cells and this B cell population.

Original languageEnglish
Pages (from-to)4629-4638
Number of pages10
JournalJournal of Immunology
Issue number7
StatePublished - Apr 1 2008


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