Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging

Metasebya Solomon; Brian R. White; Ralph E. Nothdruft; Walter Akers; Gail Sudlow; Adam T. Eggebrecht; Samuel Achilefu; Joseph P. Culver

doi:10.1364/BOE.2.003267

Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging

Metasebya Solomon, Brian R. White, Ralph E. Nothdruft, Walter Akers, Gail Sudlow, Adam T. Eggebrecht, Samuel Achilefu, Joseph P. Culver

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN.

Original language	English
Pages (from-to)	3267-3277
Number of pages	11
Journal	Biomedical Optics Express
Volume	2
Issue number	12
DOIs	https://doi.org/10.1364/BOE.2.003267
State	Published - 2011

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abstract = "We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN.",

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AU - Solomon, Metasebya

AU - White, Brian R.

AU - Nothdruft, Ralph E.

AU - Akers, Walter

AU - Sudlow, Gail

AU - Eggebrecht, Adam T.

AU - Achilefu, Samuel

AU - Culver, Joseph P.

PY - 2011

Y1 - 2011

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AB - We have developed a fiber-based, video-rate fluorescence diffuse optical tomography (DOT) system for noninvasive in vivo sentinel lymph node (SLN) mapping. Concurrent acquisition of fluorescence and reference signals allowed the efficient generation of ratio-metric data for 3D image reconstruction. Accurate depth localization and high sensitivity to fluorescent targets were established in to depths of >10 mm. In vivo accumulation of indocyanine green (ICG) dye was imaged in the region of the SLN following intradermal injection into the forepaw of rats. These results suggest that video-rate fluorescence DOT has significant potential as a clinical tool for noninvasive mapping of SLN.

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Video-rate fluorescence diffuse optical tomography for in vivo sentinel lymph node imaging

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