Vibrational photoacoustic microscopy for depth-resolved bond-selective imaging of tissues and organisms

Han Wei Wang, Ning Chai, Song Hu, Wei Dou, David Umulis, Lihong V. Wang, Michael Sturek, Robert Lucht, Ji Xin Cheng

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

We realize vibrational photoacoustic microscopy (VPA) using molecular excitation of overtone vibration and acoustic detection of the resultant pressure transients. We demonstrate 3-D VPA imaging of lipid-rich atherosclerotic plaques and lipid bodies in living Drosophila larvae by exciting 2nd overtone of the CH bond stretch around 8300 cm1. Depth-resolved spectral analysis and a penetration depth of several mm are available.

Original languageEnglish
Title of host publication2011 Conference on Lasers and Electro-Optics
Subtitle of host publicationLaser Science to Photonic Applications, CLEO 2011
StatePublished - 2011
Event2011 Conference on Lasers and Electro-Optics, CLEO 2011 - Baltimore, MD, United States
Duration: May 1 2011May 6 2011

Publication series

Name2011 Conference on Lasers and Electro-Optics: Laser Science to Photonic Applications, CLEO 2011

Conference

Conference2011 Conference on Lasers and Electro-Optics, CLEO 2011
Country/TerritoryUnited States
CityBaltimore, MD
Period05/1/1105/6/11

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