Vascular gene expression in nonneoplastic and malignant brain

Stephen L. Madden, Brian P. Cook, Mariana Nacht, William D. Weber, Michelle R. Callahan, Yide Jiang, Michael R. Dufault, Xiaoming Zhang, Wen Zhang, Jennifer Walter-Yohrling, Cecile Rouleau, Viatcheslav R. Akmaev, Clarence J. Wang, Xiaohong Cao, Thia B. St. Martin, Bruce L. Roberts, Beverly A. Teicher, Katherine W. Klinger, Radu Virgil Stan, Brenden LuceyEleanor B. Carson-Walter, John Laterra, Kevin A. Walter

Research output: Contribution to journalArticlepeer-review

149 Scopus citations

Abstract

Malignant gliomas are uniformly lethal tumors whose morbidity is mediated in large part by the angiogenic response of the brain to the invading tumor. This profound angiogenic response leads to aggressive tumor invasion and destruction of surrounding brain tissue as well as blood-brain barrier breakdown and life-threatening cerebral edema. To investigate the molecular mechanisms governing the proliferation of abnormal microvasculature in malignant brain tumor patients, we have undertaken a cell-specific transcriptome analysis from surgically harvested nonneoplastic and tumor-associated endothelial cells. SAGE-derived endothelial cell gene expression patterns from glioma and nonneoplastic brain tissue reveal distinct gene expression patterns and consistent up-regulation of certain glioma endothelial marker genes across patient samples. We define the G-protein-coupled receptor RDC1 as a tumor endothelial marker whose expression is distinctly induced in tumor endothelial cells of both brain and peripheral vasculature. Further, we demonstrate that the glioma-induced gene, PV1, shows expression both restricted to endothelial cells and coincident with endothelial cell tube formation. As PV1 provides a framework for endothelial cell caveolar diaphragms, this protein may serve to enhance glioma-induced disruption of the blood-brain barrier and transendothelial exchange. Additional characterization of this extensive brain endothelial cell gene expression database will provide unique molecular insights into vascular gene expression.

Original languageEnglish
Pages (from-to)601-608
Number of pages8
JournalAmerican Journal of Pathology
Volume165
Issue number2
DOIs
StatePublished - Aug 2004

Fingerprint Dive into the research topics of 'Vascular gene expression in nonneoplastic and malignant brain'. Together they form a unique fingerprint.

Cite this