Validation of Dynamic Contrast-Enhanced Ultrasound in Rodent Kidneys as an Absolute Quantitative Method for Measuring Blood Perfusion

Paul Kogan, Kennita A. Johnson, Steven Feingold, Nicholas Garrett, Ismayil Guracar, William J. Arendshorst, Paul A. Dayton

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Contrast-enhanced ultrasound (CEUS) has demonstrated utility in the monitoring of blood flow in tissues, organs and tumors. However, current CEUS methods typically provide only relative image-derived measurements, rather than quantitative values of blood flow in milliliters/minute per gram of tissue. In this study, CEUS derived parameters of blood flow are compared with absolute measurements of blood flow in rodent kidneys. Additionally, the effects of contrast agent infusion rate and transducer orientation on image-derived perfusion measurements are assessed. Both wash-in curve and time-to-refill algorithms are examined. Data illustrate that for all conditions, image-derived flow measurements were well-correlated with transit-time flow probe measurements (R > 0.9). However, we report differences in the sensitivity to flow across different transducer orientations as well as the contrast analysis algorithm utilized. Results also indicate that there exists a range of contrast agent flow rates for which image-derived estimates are consistent.

Original languageEnglish
Pages (from-to)900-908
Number of pages9
JournalUltrasound in Medicine and Biology
Volume37
Issue number6
DOIs
StatePublished - Jun 2011

Keywords

  • Blood flow
  • Contrast agent
  • Destruction-reperfusion
  • Flash replenishment
  • Microbubble
  • Perfusion

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