Validation of Dynamic Contrast-Enhanced Ultrasound in Rodent Kidneys as an Absolute Quantitative Method for Measuring Blood Perfusion

Paul Kogan; Kennita A. Johnson; Steven Feingold; Nicholas Garrett; Ismayil Guracar; William J. Arendshorst; Paul A. Dayton

doi:10.1016/j.ultrasmedbio.2011.03.011

Validation of Dynamic Contrast-Enhanced Ultrasound in Rodent Kidneys as an Absolute Quantitative Method for Measuring Blood Perfusion

Paul Kogan, Kennita A. Johnson, Steven Feingold, Nicholas Garrett, Ismayil Guracar, William J. Arendshorst, Paul A. Dayton

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Contrast-enhanced ultrasound (CEUS) has demonstrated utility in the monitoring of blood flow in tissues, organs and tumors. However, current CEUS methods typically provide only relative image-derived measurements, rather than quantitative values of blood flow in milliliters/minute per gram of tissue. In this study, CEUS derived parameters of blood flow are compared with absolute measurements of blood flow in rodent kidneys. Additionally, the effects of contrast agent infusion rate and transducer orientation on image-derived perfusion measurements are assessed. Both wash-in curve and time-to-refill algorithms are examined. Data illustrate that for all conditions, image-derived flow measurements were well-correlated with transit-time flow probe measurements (R > 0.9). However, we report differences in the sensitivity to flow across different transducer orientations as well as the contrast analysis algorithm utilized. Results also indicate that there exists a range of contrast agent flow rates for which image-derived estimates are consistent.

Original language	English
Pages (from-to)	900-908
Number of pages	9
Journal	Ultrasound in Medicine and Biology
Volume	37
Issue number	6
DOIs	https://doi.org/10.1016/j.ultrasmedbio.2011.03.011
State	Published - Jun 2011

Keywords

Blood flow
Contrast agent
Destruction-reperfusion
Flash replenishment
Microbubble
Perfusion

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10.1016/j.ultrasmedbio.2011.03.011

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title = "Validation of Dynamic Contrast-Enhanced Ultrasound in Rodent Kidneys as an Absolute Quantitative Method for Measuring Blood Perfusion",

abstract = "Contrast-enhanced ultrasound (CEUS) has demonstrated utility in the monitoring of blood flow in tissues, organs and tumors. However, current CEUS methods typically provide only relative image-derived measurements, rather than quantitative values of blood flow in milliliters/minute per gram of tissue. In this study, CEUS derived parameters of blood flow are compared with absolute measurements of blood flow in rodent kidneys. Additionally, the effects of contrast agent infusion rate and transducer orientation on image-derived perfusion measurements are assessed. Both wash-in curve and time-to-refill algorithms are examined. Data illustrate that for all conditions, image-derived flow measurements were well-correlated with transit-time flow probe measurements (R > 0.9). However, we report differences in the sensitivity to flow across different transducer orientations as well as the contrast analysis algorithm utilized. Results also indicate that there exists a range of contrast agent flow rates for which image-derived estimates are consistent.",

keywords = "Blood flow, Contrast agent, Destruction-reperfusion, Flash replenishment, Microbubble, Perfusion",

author = "Paul Kogan and Johnson, {Kennita A.} and Steven Feingold and Nicholas Garrett and Ismayil Guracar and Arendshorst, {William J.} and Dayton, {Paul A.}",

note = "Funding Information: This work was supported by NIH Research Grant HL-02334 (WJA), a Pilot Study Grant from the UNC Lineberger Cancer Center (PAD).",

year = "2011",

month = jun,

doi = "10.1016/j.ultrasmedbio.2011.03.011",

language = "English",

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AU - Johnson, Kennita A.

AU - Feingold, Steven

AU - Garrett, Nicholas

AU - Guracar, Ismayil

AU - Arendshorst, William J.

AU - Dayton, Paul A.

N1 - Funding Information: This work was supported by NIH Research Grant HL-02334 (WJA), a Pilot Study Grant from the UNC Lineberger Cancer Center (PAD).

PY - 2011/6

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N2 - Contrast-enhanced ultrasound (CEUS) has demonstrated utility in the monitoring of blood flow in tissues, organs and tumors. However, current CEUS methods typically provide only relative image-derived measurements, rather than quantitative values of blood flow in milliliters/minute per gram of tissue. In this study, CEUS derived parameters of blood flow are compared with absolute measurements of blood flow in rodent kidneys. Additionally, the effects of contrast agent infusion rate and transducer orientation on image-derived perfusion measurements are assessed. Both wash-in curve and time-to-refill algorithms are examined. Data illustrate that for all conditions, image-derived flow measurements were well-correlated with transit-time flow probe measurements (R > 0.9). However, we report differences in the sensitivity to flow across different transducer orientations as well as the contrast analysis algorithm utilized. Results also indicate that there exists a range of contrast agent flow rates for which image-derived estimates are consistent.

AB - Contrast-enhanced ultrasound (CEUS) has demonstrated utility in the monitoring of blood flow in tissues, organs and tumors. However, current CEUS methods typically provide only relative image-derived measurements, rather than quantitative values of blood flow in milliliters/minute per gram of tissue. In this study, CEUS derived parameters of blood flow are compared with absolute measurements of blood flow in rodent kidneys. Additionally, the effects of contrast agent infusion rate and transducer orientation on image-derived perfusion measurements are assessed. Both wash-in curve and time-to-refill algorithms are examined. Data illustrate that for all conditions, image-derived flow measurements were well-correlated with transit-time flow probe measurements (R > 0.9). However, we report differences in the sensitivity to flow across different transducer orientations as well as the contrast analysis algorithm utilized. Results also indicate that there exists a range of contrast agent flow rates for which image-derived estimates are consistent.

KW - Blood flow

KW - Contrast agent

KW - Destruction-reperfusion

KW - Flash replenishment

KW - Microbubble

KW - Perfusion

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Validation of Dynamic Contrast-Enhanced Ultrasound in Rodent Kidneys as an Absolute Quantitative Method for Measuring Blood Perfusion

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Keywords

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