@article{98f8ca3b58974e99b1850a1ff0afc3fa,
title = "Vaccination with FimH adhesin protects cynomolgus monkeys from colonization and infection by uropathogenic Escherichia coli",
abstract = "Escherichia coli FimH adhesin mediates binding to the bladder mucosa. In mice, a FimH vaccine protects against bacterial challenge. In this study, 4 monkeys were inoculated with 100 μg of FimCH adhesin-chaperone complex mixed with MF59 adjuvant, and 4 monkeys were given adjuvant only intramuscularly. After 2 doses (day 0 and week 4), a booster at 48 weeks elicited a strong IgG antibody response to FimH in the vaccinated monkeys. All 8 monkeys were challenged with 1 mL of 108 E. coli cystitis isolate NU14. Three of the 4 vaccinated monkeys were protected from bacteruria and pyuria; all control monkeys were infected. These findings suggest that a vaccine based on the FimH adhesin of E. coli type 1 pili may have utility in preventing cystitis in humans.",
author = "Solomon Langermann and Roland M{\"o}llby and Burlein, {Jeanne E.} and Palaszynski, {Susan R.} and Auguste, {C. Gale} and Anthony DeFusco and Robert Strouse and Schenerman, {Mark A.} and Hultgren, {Scott J.} and Pinkner, {Jerome S.} and Jan Winberg and Lena Guldevall and Mats S{\"o}derh{\"a}ll and Kiyohito Ishikawa and Staffan Normark and Scott Koenig",
note = "Funding Information: Received 6 July 1999; revised 22 October 1999; electronically published 9 February 2000. All experiments were approved by the local ethics committee for animal experiments. S.J.H. and Washington University have a financial interest in Med-Immune. Financial support: NIH (grants AI-29549, DK-51406); Karolinska Institute research fund (to R.M.). Reprints or correspondence: Dr. Solomon Langermann, MedImmune, Inc., 35 W. Watkins Mill Rd., Gaithersburg, MD 20878 (langermanns @medimmune.com).",
year = "2000",
doi = "10.1086/315258",
language = "English",
volume = "181",
pages = "774--778",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
number = "2",
}