Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model

Koushik Roy; David Hamilton; Marguerite M. Ostmann; James M. Fleckenstein

doi:10.1016/j.vaccine.2009.05.076

Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model

Koushik Roy, David Hamilton, Marguerite M. Ostmann, James M. Fleckenstein

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.

Original language	English
Pages (from-to)	4601-4608
Number of pages	8
Journal	Vaccine
Volume	27
Issue number	34
DOIs	https://doi.org/10.1016/j.vaccine.2009.05.076
State	Published - Jul 23 2009

Keywords

Adhesins, Bacterial
Diarrhea
Escherichia coli
Escherichia coli Vaccines
Flagellin

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10.1016/j.vaccine.2009.05.076

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title = "Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model",

abstract = "Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.",

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AU - Roy, Koushik

AU - Hamilton, David

AU - Ostmann, Marguerite M.

AU - Fleckenstein, James M.

PY - 2009/7/23

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AB - Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.

KW - Adhesins, Bacterial

KW - Diarrhea

KW - Escherichia coli

KW - Escherichia coli Vaccines

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Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model

Abstract

Keywords

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