Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model

Koushik Roy, David Hamilton, Marguerite M. Ostmann, James M. Fleckenstein

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.

Original languageEnglish
Pages (from-to)4601-4608
Number of pages8
JournalVaccine
Volume27
Issue number34
DOIs
StatePublished - Jul 23 2009

Keywords

  • Adhesins, Bacterial
  • Diarrhea
  • Escherichia coli
  • Escherichia coli Vaccines
  • Flagellin

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