TY - JOUR
T1 - Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model
AU - Roy, Koushik
AU - Hamilton, David
AU - Ostmann, Marguerite M.
AU - Fleckenstein, James M.
PY - 2009/7/23
Y1 - 2009/7/23
N2 - Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.
AB - Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.
KW - Adhesins, Bacterial
KW - Diarrhea
KW - Escherichia coli
KW - Escherichia coli Vaccines
KW - Flagellin
UR - http://www.scopus.com/inward/record.url?scp=67649484321&partnerID=8YFLogxK
U2 - 10.1016/j.vaccine.2009.05.076
DO - 10.1016/j.vaccine.2009.05.076
M3 - Article
C2 - 19523914
AN - SCOPUS:67649484321
VL - 27
SP - 4601
EP - 4608
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 34
ER -