Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model

Koushik Roy; David Hamilton; Marguerite M. Ostmann; James M. Fleckenstein

doi:10.1016/j.vaccine.2009.05.076

Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model

Koushik Roy, David Hamilton, Marguerite M. Ostmann, James M. Fleckenstein

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.

Original language	English
Pages (from-to)	4601-4608
Number of pages	8
Journal	Vaccine
Volume	27
Issue number	34
DOIs	https://doi.org/10.1016/j.vaccine.2009.05.076
State	Published - Jul 23 2009

Keywords

Adhesins, Bacterial
Diarrhea
Escherichia coli
Escherichia coli Vaccines
Flagellin

Access to Document

10.1016/j.vaccine.2009.05.076

Cite this

@article{6c69ac9e67644995b921a4502a9dd518,

title = "Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model",

abstract = "Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.",

keywords = "Adhesins, Bacterial, Diarrhea, Escherichia coli, Escherichia coli Vaccines, Flagellin",

author = "Koushik Roy and David Hamilton and Ostmann, {Marguerite M.} and Fleckenstein, {James M.}",

year = "2009",

month = jul,

day = "23",

doi = "10.1016/j.vaccine.2009.05.076",

language = "English",

volume = "27",

pages = "4601--4608",

journal = "Vaccine",

issn = "0264-410X",

number = "34",

}

TY - JOUR

T1 - Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model

AU - Roy, Koushik

AU - Hamilton, David

AU - Ostmann, Marguerite M.

AU - Fleckenstein, James M.

PY - 2009/7/23

Y1 - 2009/7/23

N2 - Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.

AB - Enterotoxigenic Escherichia coli (ETEC) remain a leading cause diarrheal illness, prompting a search for vaccine targets that led to the recent discovery of EtpA, a secreted adhesin of ETEC that acts by bridging flagella and host cells. In a murine model, immunization with recombinant EtpA glycoprotein inhibited colonization by two EtpA-producing human ETEC strains, H10407 and E24377A. In addition, vaccination with recombinant flagellin (serotype H11) generated antibodies that specifically recognized the tips of flagella from E24377A expressing a heterologous flagellar serotype (H28) and afforded significant protection against colonization. EtpA and/or flagellin could be valuable subunit antigens in the formulation of a broadly protective ETEC vaccine.

KW - Adhesins, Bacterial

KW - Diarrhea

KW - Escherichia coli

KW - Escherichia coli Vaccines

KW - Flagellin

UR - http://www.scopus.com/inward/record.url?scp=67649484321&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2009.05.076

DO - 10.1016/j.vaccine.2009.05.076

M3 - Article

C2 - 19523914

AN - SCOPUS:67649484321

SN - 0264-410X

VL - 27

SP - 4601

EP - 4608

JO - Vaccine

JF - Vaccine

IS - 34

ER -

Vaccination with EtpA glycoprotein or flagellin protects against colonization with enterotoxigenic Escherichia coli in a murine model

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this