TY - JOUR
T1 - Utility of DNA Microarrays for Detection of Viruses in Acute Respiratory Tract Infections in Children
AU - Chiu, Charles Y.
AU - Urisman, Anatoly
AU - Greenhow, Tara L.
AU - Rouskin, Silvi
AU - Yagi, Shigeo
AU - Schnurr, David
AU - Wright, Carolyn
AU - Drew, W. Lawrence
AU - Wang, David
AU - Weintrub, Peggy S.
AU - DeRisi, Joseph L.
AU - Ganem, Don
N1 - Funding Information:
Supported by a Genentech Graduate Fellowship (A.U.) and grants from the Glaser Pediatric Network (T.G.), the Sandler Program for Asthma Research (J.D.), the Howard Hughes Medical Institute (J.D. and D.G.), and the Doris Duke Charitable Foundation (C.C., J.D., and D.G.).
PY - 2008/7
Y1 - 2008/7
N2 - Objective: To assess the utility of a panviral DNA microarray platform (Virochip) in the detection of viruses associated with pediatric respiratory tract infections (RTIs). Study design: The Virochip was compared with conventional direct fluorescent antibody (DFA)- and polymerase chain reaction (PCR)-based testing for the detection of respiratory viruses in 278 consecutive nasopharyngeal aspirate samples from 222 children. Results: The Virochip was superior in performance to DFA, showing a 19% increase in the detection of 7 respiratory viruses included in standard DFA panels, and was similar to virus-specific PCR (sensitivity, 85% to 90%; specificity, ≥99%; positive predictive value, 94% to 96%; negative predictive value, 97% to 98%) in the detection of respiratory syncytial virus, influenza A, and rhinoviruses/enteroviruses. The Virochip also detected viruses not routinely tested for or missed by DFA and PCR, as well as double infections and infections in critically ill patients that DFA failed to detect. Conclusions: Given its favorable sensitivity and specificity profile and expanded spectrum for detection, microarray-based viral testing holds promise for clinical diagnosis of pediatric RTIs.
AB - Objective: To assess the utility of a panviral DNA microarray platform (Virochip) in the detection of viruses associated with pediatric respiratory tract infections (RTIs). Study design: The Virochip was compared with conventional direct fluorescent antibody (DFA)- and polymerase chain reaction (PCR)-based testing for the detection of respiratory viruses in 278 consecutive nasopharyngeal aspirate samples from 222 children. Results: The Virochip was superior in performance to DFA, showing a 19% increase in the detection of 7 respiratory viruses included in standard DFA panels, and was similar to virus-specific PCR (sensitivity, 85% to 90%; specificity, ≥99%; positive predictive value, 94% to 96%; negative predictive value, 97% to 98%) in the detection of respiratory syncytial virus, influenza A, and rhinoviruses/enteroviruses. The Virochip also detected viruses not routinely tested for or missed by DFA and PCR, as well as double infections and infections in critically ill patients that DFA failed to detect. Conclusions: Given its favorable sensitivity and specificity profile and expanded spectrum for detection, microarray-based viral testing holds promise for clinical diagnosis of pediatric RTIs.
UR - http://www.scopus.com/inward/record.url?scp=47649120745&partnerID=8YFLogxK
U2 - 10.1016/j.jpeds.2007.12.035
DO - 10.1016/j.jpeds.2007.12.035
M3 - Article
C2 - 18571541
AN - SCOPUS:47649120745
SN - 0022-3476
VL - 153
SP - 76-83.e1
JO - Journal of Pediatrics
JF - Journal of Pediatrics
IS - 1
ER -