Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse

Paul C. Bridgman

doi:10.1007/978-1-4939-6448-2_13

Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse

Research output: Contribution to journal › Article › peer-review

Abstract

Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.

Original language	English
Pages (from-to)	185-194
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1493
DOIs	https://doi.org/10.1007/978-1-4939-6448-2_13
State	Published - 2017

Keywords

Cytoskeleton
Electron microscopy
Growth cones
Rotary shadowing
Semaphorin

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10.1007/978-1-4939-6448-2_13

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title = "Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse",

abstract = "Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.",

keywords = "Cytoskeleton, Electron microscopy, Growth cones, Rotary shadowing, Semaphorin",

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note = "Funding Information: This work was supported by NIH grant; contract grant number: NS026150 Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",

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PY - 2017

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AB - Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.

KW - Cytoskeleton

KW - Electron microscopy

KW - Growth cones

KW - Rotary shadowing

KW - Semaphorin

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JF - Methods in molecular biology (Clifton, N.J.)

ER -

Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse

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