Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse

Research output: Contribution to journalArticle

Abstract

Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.

Original languageEnglish
Pages (from-to)185-194
Number of pages10
JournalMethods in Molecular Biology
Volume1493
DOIs
StatePublished - Jan 1 2017

Keywords

  • Cytoskeleton
  • Electron microscopy
  • Growth cones
  • Rotary shadowing
  • Semaphorin

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