Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse

Paul C. Bridgman

doi:10.1007/978-1-4939-6448-2_13

Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse

Paul C. Bridgman

Research output: Contribution to journal › Article › peer-review

Abstract

Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.

Original language	English
Pages (from-to)	185-194
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1493
DOIs	https://doi.org/10.1007/978-1-4939-6448-2_13
State	Published - 2017

Keywords

Cytoskeleton
Electron microscopy
Growth cones
Rotary shadowing
Semaphorin

Access to Document

10.1007/978-1-4939-6448-2_13

Cite this

@article{90325b5163434be68acf557a4f538547,

title = "Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse",

abstract = "Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.",

keywords = "Cytoskeleton, Electron microscopy, Growth cones, Rotary shadowing, Semaphorin",

author = "Bridgman, {Paul C.}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",

year = "2017",

doi = "10.1007/978-1-4939-6448-2_13",

language = "English",

volume = "1493",

pages = "185--194",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

}

TY - JOUR

T1 - Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse

AU - Bridgman, Paul C.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2017.

PY - 2017

Y1 - 2017

N2 - Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.

AB - Rotary shadow electron microscopy (EM) of growth cone cytoskeletons provides a high-resolution method for detecting both global and macromolecular changes in cytoskeletal organization or structure. This approach can be used to study responses to repulsive guidance factors such as semaphorin 3A. Here I describe the procedures used to prepare cultured neurons for rotary-shadow EM, allowing detailed comparisons of cytoskeletal structure.

KW - Cytoskeleton

KW - Electron microscopy

KW - Growth cones

KW - Rotary shadowing

KW - Semaphorin

UR - http://www.scopus.com/inward/record.url?scp=84994372803&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-6448-2_13

DO - 10.1007/978-1-4939-6448-2_13

M3 - Article

C2 - 27787851

AN - SCOPUS:84994372803

SN - 1064-3745

VL - 1493

SP - 185

EP - 194

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Using rotary shadow electron microscopy to characterize semaphorin-mediated growth cone collapse

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this