@inbook{4710216e0dbf4d3db2534c884e612b90,
title = "Using BioID to Characterize the RAS Interactome",
abstract = "Identifying the proteins that associate with RAS oncoproteins has great potential, not only to elucidate how these mutant proteins are regulated and signal but also to identify potential therapeutic targets. Here we describe a detailed protocol to employ proximity labeling by the BioID methodology, which has the advantage of capturing weak or transient interactions, to identify in an unbiased manner those proteins within the immediate vicinity of oncogenic RAS proteins.",
keywords = "BioID, BirA, HRAS, Interactome, KRAS, NRAS, Proximity labeling",
author = "Hema Adhikari and Counter, {Christopher M.}",
note = "Funding Information: This work was supported by R01CA123031 and P01CA203657 (C.M.C.) and by a Pancreatic Cancer Action Network-NCI Fredrick National Laboratory for Cancer Research KRAS Fellowship and K99CA248495 (H.A.). Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1190-6_16",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "271--280",
booktitle = "Methods in Molecular Biology",
}