Using BioID to Characterize the RAS Interactome

Hema Adhikari, Christopher M. Counter

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations


Identifying the proteins that associate with RAS oncoproteins has great potential, not only to elucidate how these mutant proteins are regulated and signal but also to identify potential therapeutic targets. Here we describe a detailed protocol to employ proximity labeling by the BioID methodology, which has the advantage of capturing weak or transient interactions, to identify in an unbiased manner those proteins within the immediate vicinity of oncogenic RAS proteins.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages10
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • BioID
  • BirA
  • HRAS
  • Interactome
  • KRAS
  • NRAS
  • Proximity labeling


Dive into the research topics of 'Using BioID to Characterize the RAS Interactome'. Together they form a unique fingerprint.

Cite this