Using BioID to Characterize the RAS Interactome

Hema Adhikari, Christopher M. Counter

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

Identifying the proteins that associate with RAS oncoproteins has great potential, not only to elucidate how these mutant proteins are regulated and signal but also to identify potential therapeutic targets. Here we describe a detailed protocol to employ proximity labeling by the BioID methodology, which has the advantage of capturing weak or transient interactions, to identify in an unbiased manner those proteins within the immediate vicinity of oncogenic RAS proteins.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages271-280
Number of pages10
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2262
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • BioID
  • BirA
  • HRAS
  • Interactome
  • KRAS
  • NRAS
  • Proximity labeling

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