Use of stable isotopically labeled tracers to measure very low density lipoprotein-triglyceride turnover

Bruce W. Patterson, Bettina Mittendorfer, Nizar Elias, Raj Satyanarayana, Samuel Klein

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Tracer methods for VLDL-TG kinetics vary in their ability to account for the effect of tracer recycling, which can influence the calculation of VLDL-TG fractional catabolic rates (FCRs). We evaluated a novel approach, involving stable isotopically labeled glycerol or palmitate tracers in conjunction with compartmental modeling, for measuring VLDL-TG kinetics in normolipidemic human subjects. When administered as a bolus simultaneously, both tracers provided identical VLDL-TG FCRs when the data were analyzed by a compartmental model that accounted for hepatic lipid tracer recycling, but not by non-compartmental analysis. The model-derived FCR was greater than that determined using a non-compartmental approach, and was 2- to 3-fold higher than that usually reported by using a bolus of radioactive [3H]glycerol. When palmitate tracer was given as a constant infusion, VLDL-TG turnover appeared 5-fold slower, because tracer recycling through hepatic lipid pools could not be resolved with the infusion protocol. We conclude that accounting for tracer recycling, particularly the contribution of hepatic glycerolipid pools, is essential to accurately measure VLDL-TG kinetics, and that bolus injection of stable isotopically labeled glycerol or palmitate tracers in conjunction with compartmental modeling analysis offers a reliable approach for measuring VLDL-TG kinetics.

Original languageEnglish
Pages (from-to)223-233
Number of pages11
JournalJournal of lipid research
Volume43
Issue number2
StatePublished - 2002

Keywords

  • Compartmental modeling
  • Fractional catabolic rate
  • Liver
  • Non-compartmental modeling
  • Tracer kinetics
  • Tracer recycling

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