Use of recombinant Onchocerca volvulus antigens for diagnosis and surveillance of human onchocerciasis

R. Chandrashekar, A. F. Ogunrinade, G. J. Weil

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The diagnostic value of ELISAs based on recombinant Onchocerca volvulus antigens OC 3.6 and OC 9.3 was evaluated with sera from endemic areas in West Africa, Guatemala and Ecuador. IgG assays were slightly more sensitive than those that detected IgG4, and the antigen combination was significantly more sensitive than either antigen alone (OC 3.6, 93%; OC 9.3, 84%, combined 98%). These assays were also evaluated with sera from 2 villages in the Onchocerciasis Control Programme area of West Africa including one village (Pendie) with recent recrudescence of infection and one (Niarba) where transmission had been interrupted for IS years by vector control. The OC 3.6 IgG antibody assay was sensitive for new infections and exposure in Pendie; 24/24 (100%) of people with positive skin snips and 15/74 (20%) of sera from MF negative people had IgG antibodies to this antigen. In addition, antibodies to OC 3.6 often preceded the onset of skin snip positivity in Pendie. In contrast, IgG antibodies to OC 3.6 and OC 9.3 were rarely seen in children born during the IS years since transmission was interrupted by vector control in Niarba. These encouraging results suggest that antibody assays based on OC 3.6 and OC 3.3 may be valuable tools for surveillance of onchocerciasis and also for monitoring the efficacy of control programmes.

Original languageEnglish
Pages (from-to)575-580
Number of pages6
JournalTropical Medicine and International Health
Volume1
Issue number5
DOIs
StatePublished - 1996

Keywords

  • Diagnosis
  • ELISA
  • Onchocerca
  • Onchocerciasis
  • Recombinant antigen
  • Surveillance

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