@article{16b5df99c39f44539a50cb6273846e77,
title = "Use of quantitative 16S rRNA PCR to determine bacterial load does not augment conventional cerebrospinal fluid (CSF) cultures among children undergoing treatment for CSF shunt infection",
abstract = "The aim of this study was to develop a quantitative 16S rRNA assay for determination of bacterial nucleic acid load in cerebrospinal fluid (CSF) shunt infection and to compare quantitative 16S rRNA polymerase chain reaction (PCR) findings to those of conventional bacterial culture in patients treated for CSF shunt infection. We developed a quantitative 16S rRNA PCR assay that detected bacterial load across a range of 2.5 × 109 down to 2.5 × 104 16S copies/mL CSF under experimental conditions for numerous Gram-positive and Gram-negative organisms. However, when applied to archived CSF samples from 25 shunt infection episodes, correlations between positive bacterial culture and 16S rRNA levels were seen in only half of infections, and 16S rRNA levels dropped precipitously after an initial peak on the first day of sample collection. Bacterial load measured using 16S rRNA PCR does not provide sufficient information beyond bacterial culture to inform CSF shunt infection treatment.",
keywords = "Bacterial load, Cerebrospinal, Children, Infection, Shunt",
author = "Simon, {Tamara D.} and {Van Yserloo}, Brian and Kevin Nelson and David Gillespie and Randy Jensen and McAllister, {James P.} and Jay Riva-Cambrin and Chris Stockmann and Daly, {Judy A.} and Blaschke, {Anne J.}",
note = "Funding Information: Financial Disclosure: This publication was supported by STARS-kids; Clinical and Translational Science Award (CTSA) pilot funds from the University of Utah [Grant UL1RR025764 from the National Center for Research Resources (NCRR) , a component of the National Institutes of Health (NIH)]; the Department of Neurosurgery at University of Utah; Seattle Children's Center for Clinical and Translational Research (CCTR); CTSA Grant Number ULI RR025014 from the NCRR ; and Diabetes and Endocrinology Research Center grant number P30DK017047 . TDS is supported by Award K23NS062900 from the National Institute of Neurological Disorders And Stroke, the Child Health Corporation of America via the Pediatric Research in Inpatient Setting Network Executive Council, and Seattle Children's CCTR. AJB is supported by 1K23AI079401 from the National Institute of Allergy and Infectious Diseases . None of the sponsors participated in design and conduct of the study; collection, management, analysis, and interpretation of the data; or preparation, review, or approval of the manuscript. Its contents are solely the responsibility of the authors and do not necessarily represent the official view of the NCRR or NIH. ",
year = "2014",
month = feb,
doi = "10.1016/j.diagmicrobio.2013.06.027",
language = "English",
volume = "78",
pages = "188--195",
journal = "Diagnostic Microbiology and Infectious Disease",
issn = "0732-8893",
number = "2",
}