Use of manipulated stem cells for prenatal therapy

Jessica L. Roybal, Pablo Laje, Jesse D. Vrecenak, Alan W. Flake

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Scopus citations

Abstract

Prenatal stem cell therapy has broad potential for therapeutic application. "Stem cells" of interest include multipotent adult-derived stem cells, cord blood, amniotic fluid, or fetal stem cells, and embryonic or induced pluripotent stem cells. Potential manipulations of stem cells prior to their administration may include harvest, processing, enrichment, expansion, and genetic transduction. A complete description of the methodology related to all of the above is well beyond the scope of this chapter. In the interest of practical application and proven efficacy, we limit our description to adult-derived hematopoietic stem cells (HSCs) and their application to in utero transplantation with or without HSC-targeted gene transfer.

Original languageEnglish
Title of host publicationPrenatal Gene Therapy
Subtitle of host publicationConcepts, Methods, and Protocols
PublisherHumana Press Inc.
Pages169-181
Number of pages13
ISBN (Print)9781617798726
DOIs
StatePublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
Volume891
ISSN (Print)1064-3745

Keywords

  • Gene transfer
  • Hematopoietic stem cell
  • In utero transplantation
  • Lentiviral vector

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