Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803

Brian P. Landry; Jana Stöckel; Himadri B. Pakrasi

doi:10.1128/AEM.03741-12

Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803

Brian P. Landry, Jana Stöckel, Himadri B. Pakrasi

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steadystate fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.

Original language	English
Pages (from-to)	2833-2835
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	79
Issue number	8
DOIs	https://doi.org/10.1128/AEM.03741-12
State	Published - Apr 2013

Access to Document

10.1128/AEM.03741-12

Cite this

@article{ae91afe61c89433dbddf536eed4e6607,

title = "Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803",

abstract = "We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steadystate fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1\% to 50\% of untagged eYFP.",

author = "Landry, \{Brian P.\} and Jana St{\"o}ckel and Pakrasi, \{Himadri B.\}",

year = "2013",

month = apr,

doi = "10.1128/AEM.03741-12",

language = "English",

volume = "79",

pages = "2833--2835",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

number = "8",

}

TY - JOUR

T1 - Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803

AU - Landry, Brian P.

AU - Stöckel, Jana

AU - Pakrasi, Himadri B.

PY - 2013/4

Y1 - 2013/4

N2 - We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steadystate fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.

AB - We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steadystate fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.

UR - https://www.scopus.com/pages/publications/84876177525

U2 - 10.1128/AEM.03741-12

DO - 10.1128/AEM.03741-12

M3 - Article

C2 - 23396339

AN - SCOPUS:84876177525

SN - 0099-2240

VL - 79

SP - 2833

EP - 2835

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 8

ER -

Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803

Abstract

Access to Document

Other files and links

Fingerprint

Cite this