Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803

Brian P. Landry; Jana Stöckel; Himadri B. Pakrasi

doi:10.1128/AEM.03741-12

Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803

Brian P. Landry, Jana Stöckel, Himadri B. Pakrasi

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25 Scopus citations

Abstract

We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steadystate fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.

Original language	English
Pages (from-to)	2833-2835
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	79
Issue number	8
DOIs	https://doi.org/10.1128/AEM.03741-12
State	Published - Apr 2013

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title = "Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803",

abstract = "We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steadystate fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.",

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AU - Pakrasi, Himadri B.

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AB - We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steadystate fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.

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Use of degradation tags to control protein levels in the cyanobacterium Synechocystis sp. strain PCC 6803

Abstract

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