TY - JOUR
T1 - Urine and Dried Blood Spots From Children and Pregnant Women Reveal Phytochemicals, Amino Acids, and Carnitine Metabolites as Cowpea Consumption Biomarkers
AU - Tipton, Madison
AU - Baxter, Bridget A.
AU - Pfluger, Brigitte A.
AU - Sayre-Chavez, Brooke
AU - Muñoz-Amatriaín, María
AU - Broeckling, Corey D.
AU - Shani, Issah
AU - Steiner-Asiedu, Matilda
AU - Manary, Mark
AU - Ryan, Elizabeth P.
N1 - Publisher Copyright:
© 2024 Wiley-VCH GmbH.
PY - 2024/2
Y1 - 2024/2
N2 - Scope: Legumes consumption has been proven to promote health across the lifespan; cowpeas have demonstrated efficacy in combating childhood malnutrition and growth faltering, with an estimated malnutrition prevalence of 35.6% of children in Ghana. This cowpea feeding study aimed to identify a suite of metabolic consumption biomarkers in children and adults. Methods and Results: Urine and dried blood spots (DBS) from 24 children (9-21 months) and 21 pregnant women (>18 years) in Northern Ghana are collected before and after dose-escalated consumption of four cowpea varieties for 15 days. Untargeted metabolomics identified significant increases in amino acids, phytochemicals, and lipids. The carnitine metabolism pathway is represented by 137 urine and 43 DBS metabolites, with significant changes to tiglylcarnitine and acetylcarnitine. Additional noteworthy candidate biomarkers are mansouramycin C, N-acetylalliin, proline betaine, N2, N5-diacetylornithine, S-methylcysteine, S-methylcysteine sulfoxide, and cis-urocanate. S-methylcysteine and S-methylcysteine sulfoxide are targeted and quantified in urine. Conclusion: This feeding study for cowpea biomarkers supports the utility of a suite of key metabolites classified as amino acids, lipids, and phytochemicals for dietary legume and cowpea-specific food exposures of global health importance.
AB - Scope: Legumes consumption has been proven to promote health across the lifespan; cowpeas have demonstrated efficacy in combating childhood malnutrition and growth faltering, with an estimated malnutrition prevalence of 35.6% of children in Ghana. This cowpea feeding study aimed to identify a suite of metabolic consumption biomarkers in children and adults. Methods and Results: Urine and dried blood spots (DBS) from 24 children (9-21 months) and 21 pregnant women (>18 years) in Northern Ghana are collected before and after dose-escalated consumption of four cowpea varieties for 15 days. Untargeted metabolomics identified significant increases in amino acids, phytochemicals, and lipids. The carnitine metabolism pathway is represented by 137 urine and 43 DBS metabolites, with significant changes to tiglylcarnitine and acetylcarnitine. Additional noteworthy candidate biomarkers are mansouramycin C, N-acetylalliin, proline betaine, N2, N5-diacetylornithine, S-methylcysteine, S-methylcysteine sulfoxide, and cis-urocanate. S-methylcysteine and S-methylcysteine sulfoxide are targeted and quantified in urine. Conclusion: This feeding study for cowpea biomarkers supports the utility of a suite of key metabolites classified as amino acids, lipids, and phytochemicals for dietary legume and cowpea-specific food exposures of global health importance.
KW - cowpea
KW - dietary exposure biomarkers
KW - dried blood spots
KW - metabolism
KW - urine
UR - http://www.scopus.com/inward/record.url?scp=85182439419&partnerID=8YFLogxK
U2 - 10.1002/mnfr.202300222
DO - 10.1002/mnfr.202300222
M3 - Article
C2 - 38233141
AN - SCOPUS:85182439419
SN - 1613-4125
VL - 68
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
IS - 4
M1 - 2300222
ER -