TY - JOUR
T1 - Urinary concentrations of aquaporin-1 and perilipin-2 in patients with renal cell carcinoma correlate with tumor size and stage but not grade
AU - Morrissey, Jeremiah J.
AU - Mobley, Jonathan
AU - Song, Joseph
AU - Vetter, Joel
AU - Luo, Jingqin
AU - Bhayani, Sam
AU - Figenshau, R. Sherburne
AU - Kharasch, Evan D.
N1 - Funding Information:
Funding Support: Support for the present study was provided by the Department of Anesthesiology and Division of Urology, Washington University School of Medicine, a grant from the Longer Life Foundation, a grant from the Bear Cub Fund of Washington University, a grant from the Barnes-Jewish Hospital Foundation of Washington University School of Medicine (CTSA UL1 TR000448), and a grant from the National Institutes of Health (R01CA141521), each to JJM. Washington University has a patent pending for the use of urinary aquaporin-1 and perilipin-2 to diagnose kidney cancer.
PY - 2014/1
Y1 - 2014/1
N2 - Objective To evaluate the trends in urine aquaporin-1 (AQP1) and perilipin 2 (PLIN2) concentrations in patients with clear cell and papillary renal cell carcinoma (RCC), we determined the relationship between the urine concentration of these biomarkers and tumor size, grade, and stage. Materials and Methods The biomarker concentrations were determined by sensitive and specific Western blot procedures normalized to the urine creatinine excretion. The analysis included 61 patients undergoing partial or radical nephrectomy for clear cell or papillary RCC and 43 age- and sex-matched control patients. Relationships between urine biomarker concentrations and tumor size, stage, and grade were assessed. Results Patients with RCC had 35-fold and 9-fold higher median urinary AQP1 and PLIN2 concentrations, respectively, compared with controls. Both tumor markers decreased after tumor resection to concentrations equivalent to those of controls. The sensitivity and specificity were both 100% for AQP1 and 92% and 100%, respectively, for PLIN2. A significant linear correlation was found between the tumor size and the prenephrectomy AQP1 (Spearman coefficient 0.78, P <.001) and PLIN2 (Spearman coefficient 0.69, P <.001) concentrations. A correlation was found for both markers with tumor stage (overall P =.030), when the stage was dependent primarily on the tumor size (stages T1 and T2), but not with stage T3, which reflected extrarenal spread. Neither marker showed a significant correlation with tumor grade. Conclusion AQP1 and PLIN2 were significantly increased in patients with clear cell and papillary RCC compared with controls. The preoperative urinary concentrations of these markers reflected the tumor size and stage.
AB - Objective To evaluate the trends in urine aquaporin-1 (AQP1) and perilipin 2 (PLIN2) concentrations in patients with clear cell and papillary renal cell carcinoma (RCC), we determined the relationship between the urine concentration of these biomarkers and tumor size, grade, and stage. Materials and Methods The biomarker concentrations were determined by sensitive and specific Western blot procedures normalized to the urine creatinine excretion. The analysis included 61 patients undergoing partial or radical nephrectomy for clear cell or papillary RCC and 43 age- and sex-matched control patients. Relationships between urine biomarker concentrations and tumor size, stage, and grade were assessed. Results Patients with RCC had 35-fold and 9-fold higher median urinary AQP1 and PLIN2 concentrations, respectively, compared with controls. Both tumor markers decreased after tumor resection to concentrations equivalent to those of controls. The sensitivity and specificity were both 100% for AQP1 and 92% and 100%, respectively, for PLIN2. A significant linear correlation was found between the tumor size and the prenephrectomy AQP1 (Spearman coefficient 0.78, P <.001) and PLIN2 (Spearman coefficient 0.69, P <.001) concentrations. A correlation was found for both markers with tumor stage (overall P =.030), when the stage was dependent primarily on the tumor size (stages T1 and T2), but not with stage T3, which reflected extrarenal spread. Neither marker showed a significant correlation with tumor grade. Conclusion AQP1 and PLIN2 were significantly increased in patients with clear cell and papillary RCC compared with controls. The preoperative urinary concentrations of these markers reflected the tumor size and stage.
UR - http://www.scopus.com/inward/record.url?scp=84891276527&partnerID=8YFLogxK
U2 - 10.1016/j.urology.2013.09.026
DO - 10.1016/j.urology.2013.09.026
M3 - Article
C2 - 24239027
AN - SCOPUS:84891276527
SN - 0090-4295
VL - 83
SP - 256.e9-256.e14
JO - Urology
JF - Urology
IS - 1
ER -