TY - JOUR
T1 - Uracil recognition by replicative DNA polymerases is limited to the archaea, not occurring with bacteria and eukarya
AU - Wardle, Josephine
AU - Burgers, Peter M.J.
AU - Cann, Isaac K.O.
AU - Darley, Kate
AU - Heslop, Pauline
AU - Johansson, Erik
AU - Lin, Li Jung
AU - McGlynn, Peter
AU - Sanvoisin, Jonathan
AU - Stith, Carrie M.
AU - Connolly, Bernard A.
N1 - Funding Information:
J.W. is a PhD student supported by Cancer Research UK (CRUK). B.A.C. was supported by grants from UK BBSRC and the European Union. P.M.J.B. was supported, in part, by grant GM 32431 from the National Institutes of Health. E.J. was supported by the Swedish Research Council, the Swedish Cancer Society and Svenska Smärtafonden. P.M. was supported by the UK MRC and the Lister Institute of Preventive Medicine. I.K.O.C. was supported by National Science Foundation Grant MCB-023841. Funding to pay the Open Access publication charges for this article was provided by The University of Newcastle.
PY - 2008/2
Y1 - 2008/2
N2 - Family B DNA polymerases from archaea such as Pyrococcus furiosus, which live at temperatures ∼ 100°C, specifically recognize uracil in DNA templates and stall replication in response to this base. Here it is demonstrated that interaction with uracil is not restricted to hyperthermophilic archaea and that the polymerase from mesophilic Methanosarcina acetivorans shows identical behaviour. The family B DNA polymerases replicate the genomes of archaea, one of the three fundamental domains of life. This publication further shows that the DNA replicating polymerases from the other two domains, bacteria (polymerase III) and eukaryotes (polymerases δ and ε for nuclear DNA and polymerase γ for mitochondrial) are also unable to recognize uracil. Uracil occurs in DNA as a result of deamination of cytosine, either in G:C base-pairs or, more rapidly, in single stranded regions produced, for example, during replication. The resulting G:U mis-pairs/ single stranded uracils are promutagenic and, unless repaired, give rise to G:C to A:T transitions in 50% of the progeny. The confinement of uracil recognition to polymerases of the archaeal domain is discussed in terms of the DNA repair pathways necessary for the elimination of uracil.
AB - Family B DNA polymerases from archaea such as Pyrococcus furiosus, which live at temperatures ∼ 100°C, specifically recognize uracil in DNA templates and stall replication in response to this base. Here it is demonstrated that interaction with uracil is not restricted to hyperthermophilic archaea and that the polymerase from mesophilic Methanosarcina acetivorans shows identical behaviour. The family B DNA polymerases replicate the genomes of archaea, one of the three fundamental domains of life. This publication further shows that the DNA replicating polymerases from the other two domains, bacteria (polymerase III) and eukaryotes (polymerases δ and ε for nuclear DNA and polymerase γ for mitochondrial) are also unable to recognize uracil. Uracil occurs in DNA as a result of deamination of cytosine, either in G:C base-pairs or, more rapidly, in single stranded regions produced, for example, during replication. The resulting G:U mis-pairs/ single stranded uracils are promutagenic and, unless repaired, give rise to G:C to A:T transitions in 50% of the progeny. The confinement of uracil recognition to polymerases of the archaeal domain is discussed in terms of the DNA repair pathways necessary for the elimination of uracil.
UR - https://www.scopus.com/pages/publications/39449126193
U2 - 10.1093/nar/gkm1023
DO - 10.1093/nar/gkm1023
M3 - Article
C2 - 18032433
AN - SCOPUS:39449126193
SN - 0305-1048
VL - 36
SP - 705
EP - 711
JO - Nucleic acids research
JF - Nucleic acids research
IS - 3
ER -