More than 70 million tons of poly(ethylene terephthalate) (PET) are manufactured worldwide every year. The accumulation of PET waste has become a global pollution concern, motivating the urgent development of technologies to valorize post-consumer PET. The development of chemocatalytic and enzymatic approaches for depolymerizing PET to its corresponding monomers opens up new opportunities for PET upcycling through biological transformation. Here, we identify Rhodococcus jostii strain PET (RPET) that can directly use PET hydrolysate as a sole carbon source. We also investigate the potential of RPET to upcycle PET into value-added chemicals, using lycopene as a proof-of-concept product. Through rational metabolic engineering, we improve lycopene production by more than 500-fold over that of the wild type. In addition, we demonstrate the production of approximately 1,300 μg/L lycopene from PET by cascading this strain with PET alkaline hydrolysis. This work highlights the great potential of biological conversion as a means of achieving PET upcycling.

Original languageEnglish
Article number111908
JournalCell Reports
Issue number1
StatePublished - Jan 31 2023


  • CP: Microbiology
  • PET
  • alkaline hydrolysis
  • carotenoid
  • food inequality
  • plastic degradation
  • plastic pollution
  • plastic upcycling
  • poly(ethylene terephthalate)
  • synthetic biology
  • value-added chemical


Dive into the research topics of 'Upcycling of poly(ethylene terephthalate) to produce high-value bio-products'. Together they form a unique fingerprint.

Cite this