Unveiling the biodiversity of lipid species in Corynebacteria- characterization of the uncommon lipid families in C. glutamicum and pathogen C. striatum by mass spectrometry

Hay Yan J. Wang, Raju V.V. Tatituri, Nicholas K. Goldner, Gautam Dantas, Fong Fu Hsu

Research output: Contribution to journalArticlepeer-review

Abstract

Uncommon lipids in biotechnologically important Corynebacterium glutamicum and pathogen Corynebacterium striatum in genus Corynebacterium are isolated and identified by linear ion-trap multiple stage mass spectrometry (LIT MSn) with high resolution mass measurement. We redefined several lipid structures that were previously mis-assigned or not defined, including cytidine diphosphate diacylglycerol (CDP-DAG), glucuronosyl diacylglycerol (GlcA-DAG), (α-D-mannopyranosyl)-(1 → 4)-(α-D-glucuronyl diacyglycerol (Man-GlcA-DAG), 1-mycolyl-2-acyl-phosphatidylglycerol (MA-PG), acyl trehalose monomycolate (acyl-TMM). We also report the structures of mycolic acid, phosphatidylglycerol, phosphatidylinositol, cardiolipin, trehalose dimycolate lipids in which many isomeric structures are present. The LIT MSn approaches afford identification of the functional group, the fatty acid substituents and their regiospecificity in the molecules, revealing the biodiversities of the lipid species in two Corynebacterium strains that have played very different and important roles in human nutrition and health.

Original languageEnglish
Pages (from-to)158-169
Number of pages12
JournalBiochimie
Volume178
DOIs
StatePublished - Nov 2020

Keywords

  • CDP-Diacylglycerol
  • Lipidomics
  • Microbial lipids
  • Multiple stage linear ion-trap mass spectrometry
  • Mycolic acid-containing PG

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