Unreliability of tritiated cholesterol: Studies with [1,2-3H]cholesterol and [24,25-3H]cholesterol in humans

N. O. Davidson, E. H. Ahrens, H. L. Bradlow, D. J. McNamara, T. S. Parker, P. Samuel

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Over the past 18 months different lots of [1,2-3H]cholesterol and [24,25-3H]cholesterol were found to be radiochemically acceptable by conventional chemical and other in vitro tests, yet, when co-administered with [4-14C]cholesterol to human subjects, an abrupt fall in the 3H/14C specific activity ratio in plasma cholesterol was discovered in every case. We have concluded that all batches of [3H]cholesterol should be regarded as radiochemically unreliable unless they are shown to behave identically in all respects to [4-14C]cholesterol in appropriate in vivo assay system.

Original languageEnglish
Pages (from-to)2255-2259
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number4 I
DOIs
StatePublished - 1980

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