TY - JOUR
T1 - Unreliability of tritiated cholesterol
T2 - Studies with [1,2-3H]cholesterol and [24,25-3H]cholesterol in humans
AU - Davidson, N. O.
AU - Ahrens, E. H.
AU - Bradlow, H. L.
AU - McNamara, D. J.
AU - Parker, T. S.
AU - Samuel, P.
PY - 1980
Y1 - 1980
N2 - Over the past 18 months different lots of [1,2-3H]cholesterol and [24,25-3H]cholesterol were found to be radiochemically acceptable by conventional chemical and other in vitro tests, yet, when co-administered with [4-14C]cholesterol to human subjects, an abrupt fall in the 3H/14C specific activity ratio in plasma cholesterol was discovered in every case. We have concluded that all batches of [3H]cholesterol should be regarded as radiochemically unreliable unless they are shown to behave identically in all respects to [4-14C]cholesterol in appropriate in vivo assay system.
AB - Over the past 18 months different lots of [1,2-3H]cholesterol and [24,25-3H]cholesterol were found to be radiochemically acceptable by conventional chemical and other in vitro tests, yet, when co-administered with [4-14C]cholesterol to human subjects, an abrupt fall in the 3H/14C specific activity ratio in plasma cholesterol was discovered in every case. We have concluded that all batches of [3H]cholesterol should be regarded as radiochemically unreliable unless they are shown to behave identically in all respects to [4-14C]cholesterol in appropriate in vivo assay system.
UR - http://www.scopus.com/inward/record.url?scp=0018870603&partnerID=8YFLogxK
U2 - 10.1073/pnas.77.4.2255
DO - 10.1073/pnas.77.4.2255
M3 - Article
C2 - 6929548
AN - SCOPUS:0018870603
SN - 0027-8424
VL - 77
SP - 2255
EP - 2259
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 4 I
ER -