Unraveling the RNA modification code with mass spectrometry

Richard Lauman, Benjamin A. Garcia

Research output: Contribution to journalReview articlepeer-review

9 Scopus citations


The discovery and analysis of modifications on proteins and nucleic acids has provided functional information that has rapidly accelerated the field of epigenetics. While protein post-translational modifications (PTMs), especially on histones, have been highlighted as critical components of epigenetics, the post-transcriptional modification of RNA has been a subject of more recently emergent interest. Multiple RNA modifications have been known to be present in tRNA and rRNA since the 1960s, but the exploration of mRNA, small RNA, and inducible tRNA modifications remains nascent. Sequencing-based methods have been essential to the field by creating the first epitranscriptome maps of m6A, m5C, hm5C, pseudouridine, and inosine; however, these methods possess significant limitations. Here, we discuss the past, present, and future of the application of mass spectrometry (MS) to the study of RNA modifications. This journal is

Original languageEnglish
Pages (from-to)305-315
Number of pages11
JournalMolecular Omics
Issue number4
StatePublished - Aug 2020


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