Unraveling the architecture of classic hodgkin lymphoma one cell at a time

Aoki and colleagues have utilized single-cell RNA sequencing and imaging mass cytometry to describe the landscape of reactive, nonmalignant immune-cell populations present in classic Hodgkin lymphoma (cHL), and delineate their spatial proximity to malignant Hodgkin–Reed–Sternberg cells. From this study, they have identified a LAG3-expressing Tr1-type Treg cell population as prevalent mainly in MHC-II–negative cHL, implying a potential functional relationship underlying the differential responsiveness of MHC-II–negative versus MHC-II– positive cHLs to immunotherapy.