@article{b39d9dac3a6c4971b98e11f6f74ec80b,
title = "Universally Conserved Relationships between Nuclear Shape and Cytoplasmic Mechanical Properties in Human Stem Cells",
abstract = "The ability of cells to proliferate, differentiate, transduce extracellular signals and assemble tissues involves structural connections between nucleus and cytoskeleton. Yet, how the mechanics of these connections vary inside stem cells is not fully understood. To address those questions, we combined two-dimensional particle-Tracking microrheology and morphological measures using variable reduction techniques to measure whether cytoplasmic mechanics allow for discrimination between different human adherent stem cell types and across different culture conditions. Here we show that nuclear shape is a quantifiable discriminant of mechanical properties in the perinuclear cytoskeleton (pnCSK) of various stem cell types. Also, we find the pnCSK is a region with different mechanical properties than elsewhere in the cytoskeleton, with heterogeneously distributed locations exhibiting subdiffusive features, and which obeys physical relations conserved among various stem cell types. Finally, we offer a prospective basis to discriminate between stem cell types by coupling perinuclear mechanical properties to nuclear shape.",
author = "Lozoya, \{Oswaldo A.\} and Gilchrist, \{Christopher L.\} and Farshid Guilak",
note = "Funding Information: We thank Drs. Richard Superfine and E. Timothy O{\textquoteright}Brien at the Center for Computer Integrated Systems for Microscopy and Manipulation (CISMM) in the University of North Carolina at Chapel Hill for discussions on experimental designs and access to microrheology equipment. We also thank Peter Lawson and Daniel O{\textquoteright}Keefe for their contributions in the implementation of microrheology technologies at Duke University{\textquoteright}s Orthopaedic Research Laboratories (ORL). Microphotopatterning was performed in the Shared Materials Instrumentation Facility at Duke University. We are also immensely grateful to both Dr. Brenton D. Hoffman at Duke University and Dr. Sharon R. Lubkin at North Carolina State University for discussions on microrheology data analysis, postprocessing, and multivariate techniques, as well as their comments on earlier versions of this manuscript. Funding support for O.A.L. in stipends and training-related expenses were provided by NIH institutional training grants to Duke University (GM069331, AI007217). This research was supported in part by NIH grants to F.G.(AR050245, AR048182, AG015768, AG046927) and C.L.G. (AR065888).",
year = "2016",
month = mar,
day = "15",
doi = "10.1038/srep23047",
language = "English",
volume = "6",
journal = "Scientific reports",
issn = "2045-2322",
}