TY - CHAP
T1 - Unique central carbon metabolic pathways and novel enzymes in phototrophic bacteria revealed by integrative genomics, 13C-based metabolomics and fluxomics
AU - Tang, Kuo Hsiang
AU - Feng, Xueyang
AU - Bandyopadhyay, Anindita
AU - Pakrasi, Himadri B.
AU - Tang, Yinjie J.
AU - Blankenship, Robert E.
N1 - Funding Information:
This work was supported by Exobiology Program of NASA Grant NNX08AP62G (REB), National Science Foundation Career Grant MCB0954016 (YJT) and Department of Energy Grant DE-FC02-07ER64694 (HBP and YJT).
Publisher Copyright:
© Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg 2013.
PY - 2013
Y1 - 2013
N2 - Photosynthesis is the process to convert solar energy to biomass and biofuels, which are the only major solar energy storage means on Earth. To satisfy the increased demand for sustainable energy sources, it is essential to understand the process of solar energy storage, that is, the carbon metabolism in photosynthetic organisms. It has been well-recognized that one bottleneck of photosynthesis is carbon assimilation. In this report, we summarize our recent studies on the carbon metabolism pathways of several types of photosynthetic bacteria, including aerobic anoxygenic phototrophic proteobacteria, green sulfur bacteria, heliobacteria and cyanobacteria, using physiological studies, transcriptomics, enzyme assays, 13C-based metabolomics and fluxomics. Our studies have revealed several unique and/or significant central carbon metabolic pathways and novel enzymes that operate in these phototrophs, quantified CO2 assimilation pathways operative during mixotrophic cultivation conditions, and also suggested evolutionary links between photosynthetic and non-photosynthetic organisms.
AB - Photosynthesis is the process to convert solar energy to biomass and biofuels, which are the only major solar energy storage means on Earth. To satisfy the increased demand for sustainable energy sources, it is essential to understand the process of solar energy storage, that is, the carbon metabolism in photosynthetic organisms. It has been well-recognized that one bottleneck of photosynthesis is carbon assimilation. In this report, we summarize our recent studies on the carbon metabolism pathways of several types of photosynthetic bacteria, including aerobic anoxygenic phototrophic proteobacteria, green sulfur bacteria, heliobacteria and cyanobacteria, using physiological studies, transcriptomics, enzyme assays, 13C-based metabolomics and fluxomics. Our studies have revealed several unique and/or significant central carbon metabolic pathways and novel enzymes that operate in these phototrophs, quantified CO2 assimilation pathways operative during mixotrophic cultivation conditions, and also suggested evolutionary links between photosynthetic and non-photosynthetic organisms.
KW - (Re)-citrate synthase
KW - Citramalate pathway
KW - CO fixation
KW - Entner-Doudoroff pathway
KW - Metabolomics
KW - Reductive TCA cycle
UR - http://www.scopus.com/inward/record.url?scp=85037088041&partnerID=8YFLogxK
U2 - 10.1007/978-3-642-32034-7_71
DO - 10.1007/978-3-642-32034-7_71
M3 - Chapter
AN - SCOPUS:85037088041
T3 - Advanced Topics in Science and Technology in China
SP - 339
EP - 343
BT - Advanced Topics in Science and Technology in China
PB - Springer Science and Business Media Deutschland GmbH
ER -